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C9903 Sigma

Cholera Toxin B subunit

≥95% (SDS-PAGE), lyophilized powder

Synonym: CTB

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Properties

Related Categories Bacteria, Bacteria-derived adjuvants, Bioactive Small Molecule Alphabetical Index, Bioactive Small Molecules, C-CH,
InChI Key   YDXZSNHARVUYNM-UHFFFAOYSA-N
Quality Level   PREMIUM
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
mol wt   mol wt ~12 kDa
composition   Protein, ~5% Lowry
impurities   ≤0.5% Cholera toxin A subunit (SDS-PAGE)
solubility   H2O: 10 mg/mL
storage temp.   2-8°C

Description

Analysis Note

Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with 0.05-1 μg of Cholera toxin B subunit per mL.

Packaging

Package size based on protein content

Reconstitution

When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN3 and 1 mM sodium EDTA.

Physical form

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide, and sodium EDTA.

Application

Cholera Toxin B subunit has been used:
• for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4.
• in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells.

Biochem/physiol Actions

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

Features and Benefits

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

General description

Cholera Toxin is a heterohexameric AB5 enterotoxin. Cholera Toxin B subunit is the the B subunit pentamer of cholera toxin produced by Vibrio cholera. It is generally involved in the cell trafficking and signalling machineries.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

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The spinal cord is the information highway system for the brain. And axons are the road that allows information data to be transferred. So, when a traumatic spinal cord injury (SCI) occurs and axons ...
Keywords: Catalysis, Cell culture, Degradations, High performance liquid chromatography, Immunofluorescence, PAGE

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Transmission of an extracellular signal across the cell membrane is initiated by activation of many types of hormone and neurotransmitter receptors, such as G-protein coupled receptors (GPCRs), and c...
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Peer-Reviewed Papers
15

References

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