• USA Home
  • C9972 - Cholera Toxin B subunit

EMAIL THIS PAGE TO A FRIEND
C9972 Sigma

Cholera Toxin B subunit

biotin conjugate, lyophilized powder

Synonym: CTB

Purchase

Properties

Related Categories Bacteria, Bioactive Small Molecule Alphabetical Index, Bioactive Small Molecules, C-CH, Cell Biology,
InChI Key   YDXZSNHARVUYNM-UHFFFAOYSA-N
form   lyophilized powder
mol wt   mol wt ~12 kDa
composition   Protein, ~40% Lowry
conjugate   biotin conjugate
storage temp.   2-8°C

Description

Analysis Note

Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding is achieved with 0.02-1 μg of Cholera toxin B subunit-biotin conjugate per mL. The conjugated B subunit gives a similar value for 50% binding to that of unconjugated B subunit from which it is prepared.

Packaging

0.5 mg in serum bottle

Quality

Biotin content ~1.0 mole/mole protein.

Physical form

Lyophilized powder containing sodium phosphate buffer salts, sodium azide and sodium EDTA.

Biochem/physiol Actions

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

Price and Availability


DISCOVER Bioactive Small Molecules

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?