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CA200 Sigma

cAMP Enzyme Immunoassay Kit, Direct

sufficient for 96 assays

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Description

Application

Please refer to the attached Protocolfor details.

Features and Benefits

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General description

Non-radioactive, competitive immunoassay for the quantitation of total cAMP in tissue and cell cultures treated with .1M HCL.The kit cAMP uses a cAMP polyclonal antibody to competitively bind the cAMP in the sample or alkaline phosphatase moleule that has cAMP covalently attachd to it.

Other Notes

Allow reagents to warm at room temperature for 30 minutes prior to opening.

Price and Availability


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All labs need water

Standard component

Description

Product #

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0.1 M Hydrochloric Acid 1 x 30 mL SDS    
Acetic Anhydride 1 x 1 mL SDS    
cAMP-Alkaline Phosphatase Conjugate 1 x 5 mL SDS    
cAMP EIA Antibody Rabbit Anti-cAMP 1 x 5 mL SDS    
5 Cycle Logit-Log Paper 1 x 1 sheets SDS    
Cyclic AMP Standard 1 x 0.5 mL SDS    
Direct cAMP Assay Layout Sheet 1 x 1 sheets SDS    
Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea SDS    
Neutralizing Reagent 1 x 6 mL SDS    
p-Nitrophenyl Phosphate Substrate Solution 1 x 20 mL SDS    
Plate Sealer 1 ea SDS    
Stop Solution 1 x 5 mL SDS    
Triethylamine 1 x 2 mL SDS    
Wash Buffer Concentrate 1 x 30 mL SDS    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9
Flash Point(F) 
120.2 °F
Flash Point(C) 
49 °C
Protocols & Articles

Protocols

ELISA Protocols

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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