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CA201 Sigma

cAMP Enzyme Immunoassay Kit

sufficient for 96 assays

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Description

Features and Benefits

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Application

Non-radioactive, competitive immunoassay for the quantitation of cAMP in biological fluids (serum, plasma, and saliva), tissue culture media samples, or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cAMP to competitively bind cAMP or cAMP which has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the additon of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cAMP present in the well.

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Standard component

Description

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Acetic Anhydride 1 x 1 mL SDS    
Assay Buffer 2 1 x 30 mL SDS    
cAMP-Alkaline Phosphatase Conjugate 1 x 5 mL SDS    
cAMP Assay Layout Sheet 1 x 1 sheets SDS    
cAMP EIA Antibody Rabbit Anti-cAMP 1 x 5 mL SDS    
5 Cycle Logit-Log Paper 1 x 1 sheets SDS    
Cyclic AMP Standard 1 x 0.5 mL SDS    
Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea SDS    
p-Nitrophenyl Phosphate Substrate Solution 1 x 20 mL SDS    
Plate Sealer 1 ea SDS    
Stop Solution 1 x 5 mL SDS    
Triethylamine 1 x 2 mL SDS    
Wash Buffer Concentrate 1 x 30 mL SDS    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

ELISA Protocols

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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Peer-Reviewed Papers
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References

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