• USA Home
  • CA201 - cAMP Enzyme Immunoassay Kit

EMAIL THIS PAGE TO A FRIEND
CA201 Sigma

cAMP Enzyme Immunoassay Kit

sufficient for 96 assays

Purchase

Description

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Application

Non-radioactive, competitive immunoassay for the quantitation of cAMP in biological fluids (serum, plasma, and saliva), tissue culture media samples, or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cAMP to competitively bind cAMP or cAMP which has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the additon of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cAMP present in the well.

Price and Availability


Antibody Explorer

Direct Detect Infrared Spectrometer

Discover the Milli-Q® IQ 7000 ultrapure water system

Standard component

Description

Product #

Add to Cart

Acetic Anhydride 1 x 1 mL SDS    
Assay Buffer 2 1 x 30 mL SDS    
cAMP-Alkaline Phosphatase Conjugate 1 x 5 mL SDS    
cAMP Assay Layout Sheet 1 x 1 sheets SDS    
cAMP EIA Antibody Rabbit Anti-cAMP 1 x 5 mL SDS    
5 Cycle Logit-Log Paper 1 x 1 sheets SDS    
Cyclic AMP Standard 1 x 0.5 mL SDS    
Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea SDS    
p-Nitrophenyl Phosphate Substrate Solution 1 x 20 mL SDS    
Plate Sealer 1 ea SDS    
Stop Solution 1 x 5 mL SDS    
Triethylamine 1 x 2 mL SDS    
Wash Buffer Concentrate 1 x 30 mL SDS    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

ELISA Protocols

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

Related Content

Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?