CA201 Sigma

cAMP Enzyme Immunoassay Kit

sufficient for 96 assays

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Description

Features and Benefits

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Application

Non-radioactive, competitive immunoassay for the quantitation of cAMP in biological fluids (serum, plasma, and saliva), tissue culture media samples, or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cAMP to competitively bind cAMP or cAMP which has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the additon of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cAMP present in the well.

Price and Availability

Standard component

Description

Product #

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Acetic Anhydride 1×1 mL    
Assay Buffer 2 1×30 mL    
cAMP-Alkaline Phosphatase Conjugate 1×5 mL    
cAMP Assay Layout Sheet 1×1 sheet    
cAMP EIA Antibody Rabbit Anti-cAMP 1×5 mL    
5 Cycle Logit-Log Paper 1×1 sheet    
Cyclic AMP Standard 1×0.5 mL    
Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 each    
p-Nitrophenyl Phosphate Substrate Solution 1×20 mL    
Plate Sealer 1 each    
Stop Solution 1×5 mL    
Triethylamine 1×2 mL    
Wash Buffer Concentrate 1×30 mL    

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