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CASP3C Sigma

Caspase 3 Assay Kit, Colorimetric

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Properties

Related Categories Apoptosis and Cell Cycle, Caspases, Caspases Kits, Cell Analysis, Cell Biology,
Quality Level   PREMIUM
usage   sufficient for 1,000 multiwell tests (in 96-well multiwell plates)
  sufficient for 100 standard tests (1 ml)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CASP3(836)

Description

Other Notes

Use 17 megohm water only

Unit Definition

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD-pNA per min at pH 7.4 at 25 °C.

Application

The assay can be performed in 1 mL volume and measured using a spectrophotometer or in 100 ìl volume in a 96 well plate using an ELISA reader.

General description

Caspase 3 Colorimetric Assay Kit provides the reagents needed for a quick and efficient detection of caspase 3 activity in cell lysates and in purified preparations of caspase 3.

The Caspase 3 Colorimetric Assay Kit is based on the hydrolysis of acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) by caspase 3, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline is detected at 405 nm (εmM=10.5). The concentration of the pNA released from the substrate is calculated from either the absorbance values at 405 nm or from a calibration curve prepared with pNA standards (pNA standard included with the kit).

Price and Availability


Flow Cytometry Instruments

All labs need water

Kit component only

Description

Product #

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10X Assay buffer 20 mL SDS    
Caspase 3 positive control 5 μg SDS    
Water (17-18 megaOhm) 125 mL SDS    

Standard component

Description

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Ac-DEVD-CHO inhibitor .5 mg SDS A0835
Ac-DEVD-pNA substrate 15 mg SDS A2559
p-Nitroaniline standard 1 mg SDS N2128
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9
Flash Point(F) 
415.4 °F
Flash Point(C) 
213 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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