|Related Categories||Apoptosis and Cell Cycle, Caspases, Cell Analysis, Cell Biology, Cell Signaling and Neuroscience,|
|usage||sufficient for 1,000 multiwell tests (in 96-well multiwell plates)|
|sufficient for 100 standard tests (1 ml)|
|shipped in||dry ice|
|Gene Information||human ... CASP3(836)|
Use 17 megohm water only
One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD-pNA per min at pH 7.4 at 25 °C.
The assay can be performed in 1 mL volume and measured using a spectrophotometer or in 100 ìl volume in a 96 well plate using an ELISA reader.
Caspase 3 Colorimetric Assay Kit provides the reagents needed for a quick and efficient detection of caspase 3 activity in cell lysates and in purified preparations of caspase 3.
The Caspase 3 Colorimetric Assay Kit is based on the hydrolysis of acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) by caspase 3, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline is detected at 405 nm (εmM=10.5). The concentration of the pNA released from the substrate is calculated from either the absorbance values at 405 nm or from a calibration curve prepared with pNA standards (pNA standard included with the kit).
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|10X Assay buffer 20 mL|
|Caspase 3 positive control 5 μg|
|Water (17-18 megaOhm) 125 ml|
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≥90% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous glycerol solution, >1.0 units/mg protein
≥97% (HPLC), powder
Certificate of Analysis
|Precautionary statements||P261-P273-P280-P301 + P310-P311|
|Hazard Codes (Europe)||T|
|Risk Statements (Europe)||23/24/25-33-52/53|
|Safety Statements (Europe)||36/37-45-61|
|RIDADR||UN 3316 6.1/PG 2|
|Flash Point(F)||415.4 °F|
|Flash Point(C)||213 °C|
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