Caspase 3 Assay Kit, Colorimetric
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Properties
| Related Categories | Apoptosis and Cell Cycle, Caspases, Cell Analysis, Cell Biology, Cell Signaling and Neuroscience, |
| Quality Level | PREMIUM |
| usage | sufficient for 1,000 multiwell tests (in 96-well multiwell plates) |
| sufficient for 100 standard tests (1 ml) | |
| shipped in | dry ice |
| storage temp. | −20°C |
| Gene Information | human ... CASP3(836) |
Description
Other Notes
Use 17 megohm water only
Unit Definition
One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD-pNA per min at pH 7.4 at 25 °C.
Application
The assay can be performed in 1 mL volume and measured using a spectrophotometer or in 100 ìl volume in a 96 well plate using an ELISA reader.
General description
Caspase 3 Colorimetric Assay Kit provides the reagents needed for a quick and efficient detection of caspase 3 activity in cell lysates and in purified preparations of caspase 3.
The Caspase 3 Colorimetric Assay Kit is based on the hydrolysis of acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) by caspase 3, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline is detected at 405 nm (εmM=10.5). The concentration of the pNA released from the substrate is calculated from either the absorbance values at 405 nm or from a calibration curve prepared with pNA standards (pNA standard included with the kit).
Price and Availability
Kit component only
|
Description |
Product # |
Add to Cart |
|---|---|---|
| 10X Assay buffer 20 mL | ||
| Caspase 3 positive control 5 μg | ||
| Water (17-18 megaOhm) 125 ml |
Standard component
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