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CASP3F Sigma

Caspase 3 Assay Kit, Fluorimetric



Related Categories Apoptosis and Cell Cycle, Caspases, Caspases Kits, Cell Analysis, Cell Biology,
usage   sufficient for 1,000 multiwell tests
  sufficient for 100 standard tests (2 ml)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CASP3(836)



The Caspase 3 Fluorimetric Assay Kit is designed for the detection and measurement of caspase 3 activity in cell extracts or purified preparations of caspase 3.

Unit Definition

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C.

General description

The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).

Price and Availability

All labs need water

Kit component only


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10X Assay buffer 20 mL SDS    
Caspase 3 positive control 5 μg SDS    
5X Lysis buffer 5 mL SDS    
Water (17-18 megaOhm) 125 mL SDS    

Standard component


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Ac-DEVD-AMC substrate 2.5 mg SDS A1086
Ac-DEVD-CHO inhibitor .5 mg SDS A0835
7-Amino-4-methylcoumarin standard 1 mg SDS A9891
Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Apoptosis Detection Kits

Programmed cell death is a fundamental process important in development, as well as a principle mechanism of tumor suppression. Apoptosis is triggered in non-malignant cells as a protective mechanism...
Dalit Weinstein-Fischer, Ph.D., Supervisor of Research & Development; Rina Altman, M.Sc., Scientist of Research & Development; Dorit Zharhary, Ph.D., Director of Research & Development
Biowire Spring 2012, 22–24
Keywords: Antibiotics, Apoptosis, Cancer, Condensations, Flow cytometry, Growth factors, Ligands, Methods, Phosphorylations, Separation, Transcription

Peer-Reviewed Papers


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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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