• USA Home
  • CASP3F - Caspase 3 Assay Kit, Fluorimetric

EMAIL THIS PAGE TO A FRIEND
CASP3F Sigma

Caspase 3 Assay Kit, Fluorimetric

Purchase

Properties

Related Categories Apoptosis and Cell Cycle, Caspases, Caspases Kits, Cell Analysis, Cell Biology,
usage   sufficient for 1,000 multiwell tests
  sufficient for 100 standard tests (2 ml)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CASP3(836)

Description

Application

The Caspase 3 Fluorimetric Assay Kit is designed for the detection and measurement of caspase 3 activity in cell extracts or purified preparations of caspase 3.

Unit Definition

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C.

General description

The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).

Price and Availability


Flow Cytometry Instruments

All labs need water

Kit component only

Description

Product #

Add to Cart

10X Assay buffer 20 mL SDS    
Caspase 3 positive control 5 μg SDS    
5X Lysis buffer 5 mL SDS    
Water (17-18 megaOhm) 125 mL SDS    

Standard component

Description

Product #

Add to Cart

Ac-DEVD-AMC substrate 2.5 mg SDS A1086
Ac-DEVD-CHO inhibitor .5 mg SDS A0835
7-Amino-4-methylcoumarin standard 1 mg SDS A9891
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Apoptosis Detection Kits

Programmed cell death is a fundamental process important in development, as well as a principle mechanism of tumor suppression. Apoptosis is triggered in non-malignant cells as a protective mechanism...
Dalit Weinstein-Fischer, Ph.D., Supervisor of Research & Development; Rina Altman, M.Sc., Scientist of Research & Development; Dorit Zharhary, Ph.D., Director of Research & Development
Biowire Spring 2012, 22–24
Keywords: Antibiotics, Apoptosis, Cancer, Condensations, Flow cytometry, Growth factors, Ligands, Methods, Phosphorylations, Separation, Transcription

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

P8340 Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?