|Related Categories||Apoptosis and Cell Cycle, Caspases, Cell Analysis, Cell Biology, Cell Signaling and Neuroscience,|
|usage||sufficient for 1,000 multiwell tests|
|sufficient for 100 standard tests (2 ml)|
|shipped in||dry ice|
|Gene Information||human ... CASP3(836)|
The Caspase 3 Fluorimetric Assay Kit is designed for the detection and measurement of caspase 3 activity in cell extracts or purified preparations of caspase 3.
One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C.
The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).
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|10X Assay buffer 20 mL|
|Caspase 3 positive control 5 μg|
|5X Lysis buffer 5 mL|
|Water (17-18 megaOhm) 125 ml|
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Certificate of Analysis
Certificate of Origin
Programmed cell death is a fundamental process important in development, as well as a principle mechanism of tumor suppression. Apoptosis is triggered in non-malignant cells as a protective mechanism...
Dalit Weinstein-Fischer, Ph.D., Supervisor of Research & Development; Rina Altman, M.Sc., Scientist of Research & Development; Dorit Zharhary, Ph.D., Director of Research & Development
Biowire Spring 2012, 22–24
Keywords: Antibiotics, Apoptosis, Cancer, Condensations, Flow cytometry, Growth factors, Ligands, Phosphorylations, Separation, Transcription
Cisplatin induces mitochondrial oxidative stress with resultant energetic metabolism impairment, membrane rigidification and apoptosis in rat liver Martins,N et al. J. Appl. Toxicol. 28(3), 337-344, (2008)
Zinc octa-n-alkyl phthalocyanines in photodynamic therapy: photophysical properties, accumulation and apoptosis in cell cultures, studies in erythrocytes and topical application to Balb/c mice skin Kaestner,L et al. Photochem. Photobiol. 2, 660-667, (2003)
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