Caspase 3 Assay Kit, Fluorimetric
DOWNLOAD MSDS (PDF)-
Popular Documents: Bulletin (PDF) | Specification Sheet (PDF)
Properties
| Related Categories | Apoptosis and Cell Cycle, Caspases, Cell Analysis, Cell Biology, Cell Signaling and Neuroscience, |
| usage | sufficient for 1,000 multiwell tests |
| sufficient for 100 standard tests (2 ml) | |
| shipped in | dry ice |
| storage temp. | −20°C |
| Gene Information | human ... CASP3(836) |
Description
Application
The Caspase 3 Fluorimetric Assay Kit is designed for the detection and measurement of caspase 3 activity in cell extracts or purified preparations of caspase 3.
Unit Definition
One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C.
General description
The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).
Price and Availability
Kit component only
|
Description |
Product # |
Add to Cart |
|---|---|---|
| 10X Assay buffer 20 mL | ||
| Caspase 3 positive control 5 μg | ||
| 5X Lysis buffer 5 mL | ||
| Water (17-18 megaOhm) 125 ml |
Standard component
Customers Also Viewed
≥90% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous glycerol solution, >1.0 units/mg protein
Technical Service:
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Bulk Ordering & Pricing:
Need larger quantities for your development, manufacturing or research applications?
