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CASP6F Sigma

Caspase 6 Assay Kit, Fluorimetric

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Description

General description

The Caspase 6 Fluorimetric Assay Kit is based on the hydrolysis of the peptide substrate Acetyl-Val-Glu-Ile-Asp-7-amino-4-methyl coumarin (Ac-VEID-AMC) by caspase 6 that results in the release of a 7-amino-4-methyl coumarin (AMC) moiety. The excitation and emission wavelengths of AMC are 360 nm and 440 nm, respectively. The concentration of the AMC released can be calculated from a calibration curve prepared from AMC standards (included with the kit).
The kit detects caspase 6 activity in crude and purified preparations of caspase 6.
Complete kit contains:
• purified caspase 6 for a positive control
• caspase 6 inhibitor
• AMC fluorescent standard

Quantity

The kit provides sufficient reagents for 100 tests of 100 μl in 96-well plates.

Price and Availability


Flow Cytometry Instruments

All labs need water

Standard component

Description

Product #

Add to Cart

Acetyl-Val-Glu-Ile-Asp-7-amido-4-methylcoumarin Substrate .25 mL SDS    
7-Amino-4-methylcoumarin Standard 1 mL SDS    
Caspase-6 5 μg SDS    
Safety & Documentation

Safety Information

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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