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CASP8C Sigma

Caspase 8 Assay Kit, Colorimetric

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Description

Application

The Caspase 8 Colorimetric Assay Kit is based on the hydrolysis of the peptide substrate Acetyl-Ile-Glu-Thr-Asp-p-Nitroaniline (Ac-IETD-pNA) by caspase-8 that results in the release of a p-Nitroaniline (p-NA) moiety. The p-NA is read at 405 nm. The concentration of the p-NA released can be calculated from a calibration curve prepared from p-NA standards (included with the kit).
The kit detects caspase 8 activity in crude and purified preparations of caspase 8.
Complete kit contains:
• purified caspase 8 for a positive control
• caspase 8 inhibitor
• p-NA fluorescent standard

Quantity

The kit provides sufficient reagents for 100 tests of 100 μL in 96-well plates.

Price and Availability


All labs need water

Biomedical Applications

Kit component only

Description

Product #

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Water, 17-18 megohm 125 mL SDS    

Standard component

Description

Product #

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Acetyl-Ile-Glu-Thr-Asp-Aldehyde Inhibitor 100 μL SDS    
Acetyl-Ile-Glu-Thr-Asp-p-nitroaniline Substrate 100 μL SDS    
10X Assay Buffer 5 mL SDS    
5X Lysis Buffer 5 mL SDS    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers
15

References

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