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CASP8F Sigma

Caspase 8 Assay Kit, Fluorimetric

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Description

General description

The Caspase 8 Fluorimetric Assay Kit is based on the hydrolysis of the peptide substrate Acetyl-Ile-Glu-Thr-Asp-7-amino-4-methyl coumarin (Ac-IETD-AMC) by caspase-8 that results in the release of a 7-amino-4-methyl coumarin (AMC) moiety. The excitation and emission wavelengths of AMC are 360 nm and 460 nm, respectively. The concentration of the AMC released can be calculated from a calibration curve prepared from AMC standards (included with the kit).
The kit detects caspase 8 activity in crude and purified preparations of caspase 8.
Complete kit contains:
• purified caspase 8 for a positive control
• caspase 8 inhibitor
• AMC fluorescent standard

Price and Availability


Flow Cytometry Instruments

All labs need water

Standard component

Description

Product #

Add to Cart

Acetyl-Ile-Glu-Thr-Asp-Aldehyde Inhibitor .5 mL SDS    
Acetyl-Ile-Glu-Thr-Asp-7-amido-4-methylcoumarin Substrate 1 mL SDS    
7-Amino-4-methylcoumarin Standard 1 mL SDS    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
Flash Point(F) 
185 °F
Flash Point(C) 
85 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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