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CE0050 Sigma

CelLytic MEM Protein Extraction Kit

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Description

Application

The kit offers a fast and convenient method to isolate hydrophobic and raft microdomain associated proteins from cells. The method is based on phase separation and does not require cell membrane isolation. The separated proteins can be used for further experiments such as SDS-PAGE, Western blotting, dot blotting, and immunoprecipitation. The kit has been tested on, but not limited to, HeLa, HEK-293, NIH 3T3, COS and CHO cell lines.

General description

Membrane proteins make up around 20-30% of an organism′s genome and serve as cellular gatekeepers, regulators, and sensors. They have diverse cellular functions, such as shielding the cell from external toxins, being the starting point of intracellular signaling cascades, and retaining critical ion concentrations.

Suitability

Sufficient reagents supplied for 80 tests.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


Detect Your Unseen Protein

All labs need water

Kit component also available separately

Description

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Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 1 mL SDS P8340

Kit component only

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Lysis and Separation Buffer 50 mL SDS    
Wash Buffer for CelLytic MEM 50 mL SDS    
Sodium Chloride, 4M Solution 1.5 mL SDS    
Safety & Documentation

Safety Information

Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

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