|Related Categories||Biochemicals and Reagents, Cell Biology, Cell Lysis, Cell Lysis Products, Cell Lysis and Protein Extraction,|
|shipped in||wet ice|
The kit offers a fast and convenient method to isolate hydrophobic and raft microdomain associated proteins from cells. The method is based on phase separation and does not require cell membrane isolation. The separated proteins can be used for further experiments such as SDS-PAGE, Western blotting, dot blotting, and immunoprecipitation. The kit has been tested on, but not limited to, HeLa, HEK-293, NIH 3T3, COS and CHO cell lines.
Sufficient reagents supplied for 80 tests.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
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|Lysis and Separation Buffer 50 ml|
|Wash Buffer for CelLytic MEM 50 ml|
|Sodium Chloride, 4M Solution 1.5 ml|
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Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.
For mammalian tissues
For mammalian tissue or cultured cells
for use with mammalian cell and tissue extracts, DMSO solution
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