CE0050 Sigma

CelLytic MEM Protein Extraction Kit

Description

Application

The kit offers a fast and convenient method to isolate hydrophobic and raft microdomain associated proteins from cells. The method is based on phase separation and does not require cell membrane isolation. The separated proteins can be used for further experiments such as SDS-PAGE, Western blotting, dot blotting, and immunoprecipitation. The kit has been tested on, but not limited to, HeLa, HEK-293, NIH 3T3, COS and CHO cell lines.

General description

Membrane proteins make up around 20-30% of an organism′s genome and serve as cellular gatekeepers, regulators, and sensors. They have diverse cellular functions, such as shielding the cell from external toxins, being the starting point of intracellular signaling cascades, and retaining critical ion concentrations.1

Suitability

Sufficient reagents supplied for 80 tests.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Kit component also available separately

Description

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Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 1 ml MSDS P8340

Kit component only

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Lysis and Separation Buffer 50 ml MSDS    
Wash Buffer for CelLytic MEM 50 ml MSDS    
Sodium Chloride, 4M Solution 1.5 ml MSDS    

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