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CELLYTPN1 Sigma

CelLytic PN Isolation/Extraction Kit

For plant leaves

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Description

Application

The nuclear protein extract is suitable for the detection of DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis as well as Western blot assay and similar techniques. The isolated nuclei can also be used as a source for chromatin, genomic DNA, RNA, etc. The kit provides a detailed protocol for nuclei isolation and protein extraction from four plant models: tobacco, tomato, spinach, and Arabidopsis.

General description

CelLytic-PN extraction kit is for the isolation of nuclei and extraction of functional nuclear proteins from plant leaves. Nuclei or nuclear proteins can be extracted from few grams to hundreds grams of fresh and frozen leaves. The nuclear protein extract is suitable for the detection of DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis as well as western blot assay and similar techniques. The isolated nuclei can also be used as a source for chromatin, genomic DNA and histones.

Other Notes

This kit is for the rapid isolation of nuclei and extraction of functional nuclear proteins from plant leaves. Nuclei or nuclear proteins can be extracted from a few grams to hundreds of grams of fresh and frozen leaves.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


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Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
UN 3077 9 / PGIII
Protocols & Articles
Peer-Reviewed Papers
15

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