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CG200 Sigma

cGMP Enzyme Immunoassay Kit, Direct

sufficient for 96 assays

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Description

Features and Benefits

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Application

Non-radioactive, competitive immunoassay for the quantitation of total cGMP in tissue and cell cultures. Buffer contains 0.1N HCl that aids in the lysis of cells, inhibits endogenous phosphodiesterases and stabilizes the cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the addition of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.

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0.1 M Hydrochloride Acid 1 x 30 mL SDS    
Acetic Anhydride 1 x 1 mL SDS    
cGMP-Alkaline Phosphatase Conjugate 1 x 5 mL SDS    
cGMP EIA Antibody Rabbit Anti-cGMP 1 x 5 mL SDS    
Cyclic GMP Standard 1 x 0.5 mL SDS    
Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea SDS    
Neutralizing Reagent 1 x 6 mL SDS    
p-Nitrophenyl Phosphate Substrate Solution 1 x 20 mL SDS    
Plate Sealer 1 ea SDS    
Stop Solution 1 x 5 mL SDS    
Triethylamine 1 x 2 mL SDS    
Wash Buffer Concentrate 1 x 30 mL SDS    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9
WGK Germany 
3
Flash Point(F) 
120.2 °F
Flash Point(C) 
49 °C

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

ELISA Protocols

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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