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CGP1 Sigma

Glutathione Peroxidase Cellular Activity Assay Kit

Synonym: Cellular glutathione peroxidase, c-GPx

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Properties

Related Categories Cell Analysis, Cell Biology, Cell Signaling and Neuroscience, Cell Stress, Nitric Oxide and Cell Stress,
usage   sufficient for 100 tests
shelf life   Kit is stable for 24 months when unopened.
shipped in   wet ice
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Other Notes

This kit was tested with rabbit reticulocyte lysate and with glutathione peroxidase enzyme.

General description

The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.

Price and Availability


Flow Cytometry Instruments

All labs need water

Kit component only

Description

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Glutathione Peroxidase Assay Buffer 120 mL SDS    
NADPH Assay Reagent 5 vials SDS    

Standard component

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tert-Butyl hydroperoxide 1 mL SDS    
Safety & Documentation

Safety Information

Signal word 
Danger
Precautionary statements 
RIDADR 
UN 3316 9
WGK Germany 
3
Protocols & Articles

Articles

Mitochondrial Stress and ROS

Oxidative stress is implicated in the pathogenesis of lipotoxicity in both animal and human studies. Chronic oxidative stress is linked to insulin resistance in multiple tissues. Oxidative stress is ...
BioFiles 2011, 6.4, 22.
Keywords: Anti-inflammatory agents, Apoptosis, Cancer, Cell signaling, Cellular processes, Gene expression, Indicators, Metabolism, Oxidations, Respiratory

Peer-Reviewed Papers
15

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