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CHY5S Sigma

α-Chymotrypsin from bovine pancreas

≥40 units/mg protein, vial of 5 mg

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Properties

Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates More...
description   aseptically filled
type   Type IV-S
mol wt   mol wt 25 kDa
composition   protein, ≥85% UV
packaging   vial of 5 mg
storage temp.   −20°C
Gene Information   cow ... CTRB1(618826)

Description

Analysis Note

Protein determined by A1%/280

Biochem/physiol Actions

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu2+ and Hg2+.

Unit Definition

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Application

α-Chymotrypsin from bovine has been used in a study to inform proteasome inhibition in order to advance anticancer research. α-Chymotrypsin from bovine has also been used in a study that functionalized surface anchored poly(methylhydrosiloxane) thin films on oxidized silicon wafers.

The enzyme from Sigma has been used to assess the effect of limited proteolysis with α-chymotrypsin on the sperm penetration.

Price and Availability


Laboratory Gloves

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
GC3050000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Procedure for Enzymatic Assay of Chymotrypsin (α‑Chymotrypsin, EC 3.4.21.1)

This procedure is for products with a specification for Chymotrypsin activity. It is not to be used to assay Insoluble Chymotrypsin such as Catalog No. C9134. The procedure is a continuous spectropho...
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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substrate

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A3401 Ala-Ala-Phe-7-amido-4-methylcoumarin, protease substrate
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A9273 Ala-Ala-Val-Ala p-nitroanilide, protease substrate
S1899 N-Succinyl-Gly-Gly-Phe-p-nitroanilide, protease substrate
S9761 N-Succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin, Leucine aminopeptidase substrate
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