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CKOZFN2425 Sigma

CompoZr® Knockout ZFN Kit

Human ITLN1 (NM_017625)

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Properties

Related Categories CompoZr Knockout ZFN Kits, Functional Genomics and RNAi, Human CompoZr Knockout ZFN Kits, IN-KD, Molecular Biology More...
NCBI accession no.   NM_017625
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... ITLN1(55600)

Description

General description

CompoZr Knockout ZFNs create targeted double strand breaks at a gene specific target locus. Through the cellular process of Non-Homologous End Joining, this double strand break can result in modification of the DNA sequence and therefore create a functional knockout of the targeted gene. To provide the best chance of creating a functional gene knockout, CompoZr Knockout ZFNs are targeted to the first 2/3 of the coding region for the gene of interest.

Legal Information

All Zinc Finger Nucleases are sold under license from Sangamo Biosciences. For a copy of the Label License provided with purchase of CompoZr ZFNs, please visit the ZFN Label License page.

CompoZr is a registered trademark of Sigma-Aldrich Co. LLC

Physical form

CompoZr Knockout ZFNs are provided as 10 aliquots of mRNA encoding for the ZFNs along with plasmid DNA for both ZFN pairs. Also included in the kit are custom-designed primers, that allow for determination of the rate of mutation and for screening of individual clones that have the desired mutational event, and positive control DNA that has been modified using the CompoZr Knockout ZFN.

Biochem/physiol Actions

The zinc finger nuclease (ZFN) provided in this kit targets the gene encoding omentin (intelectin-1). It has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. It increases protein kinase B (AKT) phosphorylation in the absence and presence of insulin. Omentin was suggested to play a role in the defense system against microorganisms by recognizing carbohydrate chains of pathogens and bacterial components containing galactofuranosyl residues. It has anti-inflammatory and insulin-sensitizing properties. The protein may be a biomarker of metabolic disorders. It has been shown to be over-expressed in human malignant pleural mesothelioma (MPM).

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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CKOZFND2425 CompoZr® Knockout ZFN Kit, ZFN plasmid only, Human ITLN1 (NM_017625)

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