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CKOZFND37692 Sigma

CompoZr® Knockout ZFN Kit, ZFN plasmid only

Mouse Olfr59 (NM_011002.2)

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Related Categories CompoZr Knockout ZFN Kits, Functional Genomics and RNAi, Molecular Biology, Mouse CompoZr Knockout ZFN, plasmid only, Kits, OLFR-OLFR600 More...
NCBI accession no.   NM_011002.2
shipped in   dry ice
storage temp.   −70°C
Gene Information   mouse ... Olfr59(18359)

Description

General description

CompoZr Knockout ZFNs create targeted double strand breaks at a gene specific target locus. Through the cellular process of Non-Homologous End Joining, this double strand break can result in modification of the DNA sequence and therefore create a functional knockout of the targeted gene. To provide the best chance of creating a functional gene knockout, CompoZr Knockout ZFNs are targeted to the first 2/3 of the coding region for the gene of interest.

A database for the C57BL/6J mouse strain is used to determine target sites for mouse Knockout ZFNs, for questions regarding other strains of mice please contact a Sigma Technical Sales Specialist.

Legal Information

All Zinc Finger Nucleases are sold under license from Sangamo Biosciences. For a copy of the Label License provided with purchase of CompoZr ZFNs, please visit the ZFN Label License page.

CompoZr is a registered trademark of Sigma-Aldrich Co. LLC

Physical form

CompoZr Knockout ZFNs are provided as two plasmids encoding for the gene specific zinc finger nuclease. Also included in the kit are custom-designed primers, that allow for determination of the rate of mutation and for screening of individual clones that have the desired mutational event, and positive control DNA that has been modified using the CompoZr Knockout ZFN.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles
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