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CKOZFND3995 Sigma

CompoZr® Knockout ZFN Kit, ZFN plasmid only

Human BLZF1 (NM_003666)

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Related Categories AW-C1orf99, CompoZr Knockout ZFN Kits, Functional Genomics and RNAi, Human CompoZr Knockout ZFN, plasmid only, Kits, Molecular Biology More...
NCBI accession no.   NM_003666
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... BLZF1(8548)

Description

General description

CompoZr Knockout ZFNs create targeted double strand breaks at a gene specific target locus. Through the cellular process of Non-Homologous End Joining, this double strand break can result in modification of the DNA sequence and therefore create a functional knockout of the targeted gene. To provide the best chance of creating a functional gene knockout, CompoZr Knockout ZFNs are targeted to the first 2/3 of the coding region for the gene of interest.

Legal Information

All Zinc Finger Nucleases are sold under license from Sangamo Biosciences. For a copy of the Label License provided with purchase of CompoZr ZFNs, please visit the ZFN Label License page.

CompoZr is a registered trademark of Sigma-Aldrich Co. LLC

Physical form

CompoZr Knockout ZFNs are provided as two plasmids encoding for the gene specific zinc finger nuclease. Also included in the kit are custom-designed primers, that allow for determination of the rate of mutation and for screening of individual clones that have the desired mutational event, and positive control DNA that has been modified using the CompoZr Knockout ZFN.

Biochem/physiol Actions

The ZFN (zinc finger nuclease) provided in this kit targets the BLZF1 (basic leucine zipper nuclear factor 1) gene. BLZF1 might work as a transcription factor. It is involved in epigenetic regulation by binding to DNMT3L (DNA methyltransferase 3 like) and also participates in Wnt/β-catenin signaling. The BLZF1 gene is downregulated in acute promyelocytic leukemia (APL) cell line NB4. On the other hand, it is upregulated in presence of dengue virus infection. BLZF1 also interacts with GRASP55 (Golgi reassembly-stacking protein of 55 kDa) and the GTP form of rab2. It is involved in the secretory protein transport and maintenance of the Golgi structure. This gene is downregulated in hepatocellular carcinoma. This low expression is associated with poor overall survival.

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Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
Peer-Reviewed Papers
15

References

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CKOZFN3995 CompoZr® Knockout ZFN Kit, Human BLZF1 (NM_003666)

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