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CKOZFND9227 Sigma

CompoZr® Knockout ZFN Kit, ZFN plasmid only

Human GEM (NM_005261)



Related Categories CompoZr Knockout ZFN Kits, Functional Genomics and RNAi, GAK-GO, Human CompoZr Knockout ZFN, plasmid only, Kits, Molecular Biology More...
NCBI accession no.   NM_005261
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... GEM(2669)


General description

CompoZr Knockout ZFNs create targeted double strand breaks at a gene specific target locus. Through the cellular process of Non-Homologous End Joining, this double strand break can result in modification of the DNA sequence and therefore create a functional knockout of the targeted gene. To provide the best chance of creating a functional gene knockout, CompoZr Knockout ZFNs are targeted to the first 2/3 of the coding region for the gene of interest.

Legal Information

All Zinc Finger Nucleases are sold under license from Sangamo Biosciences. For a copy of the Label License provided with purchase of CompoZr ZFNs, please visit the ZFN Label License page.

CompoZr is a registered trademark of Sigma-Aldrich Co. LLC

Physical form

CompoZr Knockout ZFNs are provided as two plasmids encoding for the gene specific zinc finger nuclease. Also included in the kit are custom-designed primers, that allow for determination of the rate of mutation and for screening of individual clones that have the desired mutational event, and positive control DNA that has been modified using the CompoZr Knockout ZFN.

Biochem/physiol Actions

The ZFN (zinc finger nuclease) provided in this kit targets the GEM (GTP binding protein overexpressed in skeletal muscle) gene. The gene encodes a guanosine triphosphate (GTP)-binding protein. It controls voltage gated Ca2+ channels and cytoskeleton dynamics. Overexpression of GEM causes disruption of stress fiber, changes in cell shape and neurite elongation in interphase cells. In addition, it is also involved in actin remodelling during mitosis. In Timothy syndrome, overexpression of GEM leads to inactivation of RhoA, thereby preventing dendritic retraction. GEM might also be associated with cell invasiveness. In HTLV-1 retrovirus (human T-cell leukemia virus type 1) infection, GEM is involved in cell-to-cell viral transmission.

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Safety Information

Safety Information for this product is unavailable at this time.
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CKOZFN9227 CompoZr® Knockout ZFN Kit, Human GEM (NM_005261)

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