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COIPP Sigma

Immunoprecipitation Vector Kit

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Properties

grade   for molecular biology
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C

Description

Components

The IP Vector Kit contains:
• pFLAG-CMV-2 Expression Vector (10 μg)
• pc-Myc-CMV-2 Expression Vector (10 μg)
• pFLAG-CMV-2-p53 control plasmid (20 μg)
• pc-Myc-CMV-2-Large T antigen control plasmid (10 μg)
• pc-Myc-CMV-2-BAP control plasmid (10 μg)
• Verification Primer-MF (2 nmol)
• Verification Primer-MR (2 nmol)

Features and Benefits

• Fast and convenient 96 well plate format
• Cleaner analysis due to strongly bound antibody
• Rapid, sensitive, quantitative results

General description

The Immunoprecipitation (IP) Vector Kit provides a unique method to validate protein-protein interactions in a convenient 96-well format. This system enables potential binding partners for the protein of interest to be tagged with ”FLAG and c-Myc epitopes, followed by capture and detection using the ANTI-FLAG M2 High Sensitivity Capture Plate.

The kit provides expression vectors ”pFLAG-CMV”-2 and pc-Myc-CMV-2 designed for the cloning and expression of protein interaction candidates as N-terminal FLAG and N-terminal c-Myc fusions. Control plasmids pFLAG-CMV-2-p53, pC-Myc-CMV-2-Large T antigen and pC-Myc-CMV-2-BAP supplied are for expression of positive and negative binding partners in the IP analysis. The kit also includes the MF-2 and MR-2 verification primers.

Vector Maps and Sequences

Price and Availability

Kit component only

Description

Product #

Add to Cart

pc-Myc-CMV-2 10 μg SDS    
pc-Myc-CMV-2-BAP 10 μg SDS    
pc-Myc-CMV-2-Large T antigen 10 μg SDS    
pFLAG-CMV™-2 10 μg SDS    
pFLAG-CMV™-2-p53 20 μg SDS    
Verification Primer-MF 2 nmol SDS    
Verification Primer-MR 2 nmol SDS    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
HG1933000
Protocols & Articles
Peer-Reviewed Papers
15

References

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