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CPISO Sigma

Chloroplast Isolation Kit

isolation of intact chloroplasts from leaves

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Description

Features and Benefits

• Specifically formulated buffer - Saves time using easy to follow simple and proven protocols
• Isolates intact chloroplasts - Useful for studies of such processes as carbon assimilation, electron flow and phosphorylation, metabolic transport or protein targeting
• Total organelle protein complement - Useful for functional studies, metabolic transport, proteomic profiling, and genomic applications
• Convenient format - Saves time and reduces waste

General description

The Chloroplast Isolation Kit provides an efficient procedure for isolating intact chloroplasts from plant leaves. The procedure involves mechanical cell wall and cell membrane breakage, removal of cell debris and leaf tissue by filtration, collection of total cell chloroplast fraction by centrifugation, and separation of intact chloroplasts from broken chloroplasts using a Percoll® layer or gradient.

Application

Chloroplast Isolation Kit may be used in chloroplast organelle research to isolate intact chloroplasts from plant leaves for structural and functional studies.

Suitability

The Chloroplast Isolation Kit has been tested for use with spinach, pea, lettuce, cabbage, mangold, and tobacco.

Price and Availability


Ultrafree Centrifugal Filter Units

Kit component also available separately

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Percoll®, pH 8.5-9.5 (25 °C), cell culture tested 100 mL SDS P4937

Kit component only

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Chloroplast Isolation Buffer 5x (CIB) 500 mL SDS    
Bovine Serum Albumin, heat shock fraction, pH 5.2, ≥96% 3 g SDS    
Chloroplast Isolation Buffer 5x (CIB) 4 ea SDS    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?