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CS0250 Sigma

p38 MAPK Activity Assay Kit

sufficient for 50 determinations

Synonym: p38 Mitogen Activated Protein Kinase Activity Assay Kit

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Properties

usage   sufficient for 50 determinations
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MAPK14(1432)

Description

Principle

The mitogen-activated protein kinase (MAPK) p38 pathway is a signaling cascade activated by proinflammatory stimuli and cellular stresses, playing a critical role in the translational regulation of proinflammatory cytokine synthesis.

Biochem/physiol Actions

The p38 MAPK Assay Kit provides an easy method to assay p38 MAPK activity and explore new p38MAPK stimuli, inhibitors, and activators. The kit provides all the reagents required for the straightforward detection and measurement of p38 MAPK activity in cell lysates, tissue homogenates, column fractions, or the purified enzyme. In addition, the kit provides a specific p38 MAPK inhibitor to enable the verification of the specificity of the kinase activity observed.
The assay is based on immunoprecipitation of the active form of p38α kinase and detection of its phosphorylation activity on the substrate ATF2 by immunoblotting, without the need for a secondary antibody. An alternative protocol for radioactive measurement of p38 MAPK activity is also provided.

Price and Availability

Kit component also available separately

Description

Product #

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Anti-p38 MAP Kinase antibody .1 mL SDS    
EZviewTM Red Protein A Affinity Gel 1 mL SDS    
ATF2 .5 mg SDS    

Kit component only

Description

Product #

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Assay Buffer For Kinase Activity 1 mL SDS    
Wash Buffer, 10X 8 mL SDS    
Monoclonal Anti-phospho-ATF2 (pThr69,71), Peroxidase conjugate 500 μg SDS    
p38 Inhibitor (SB 203580) .05 mL SDS    
Safety & Documentation

Safety Information

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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