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CS0250 Sigma

p38 MAPK Activity Assay Kit

sufficient for 50 determinations

Synonym: p38 Mitogen Activated Protein Kinase Activity Assay Kit



Related Categories Cell Analysis, Cell Biology, Cell Signaling and Neuroscience, Kinase/Phosphatase Biology, Mitogen-Activated Protein Kinase (MAPK),
usage   sufficient for 50 determinations
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MAPK14(1432)



The mitogen-activated protein kinase (MAPK) p38 pathway is a signaling cascade activated by proinflammatory stimuli and cellular stresses, playing a critical role in the translational regulation of proinflammatory cytokine synthesis.

Biochem/physiol Actions

The p38 MAPK Assay Kit provides an easy method to assay p38 MAPK activity and explore new p38MAPK stimuli, inhibitors, and activators. The kit provides all the reagents required for the straightforward detection and measurement of p38 MAPK activity in cell lysates, tissue homogenates, column fractions, or the purified enzyme. In addition, the kit provides a specific p38 MAPK inhibitor to enable the verification of the specificity of the kinase activity observed.
The assay is based on immunoprecipitation of the active form of p38α kinase and detection of its phosphorylation activity on the substrate ATF2 by immunoblotting, without the need for a secondary antibody. An alternative protocol for radioactive measurement of p38 MAPK activity is also provided.

Price and Availability

Kit component also available separately


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Anti-p38 MAP Kinase antibody .1 mL MSDS    
EZviewTM Red Protein A Affinity Gel 1 mL MSDS    
ATF2 .5 mg MSDS    

Kit component only


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Assay Buffer For Kinase Activity 1 mL MSDS    
Wash Buffer, 10X 8 mL MSDS    
Monoclonal Anti-phospho-ATF2 (pThr69,71), Peroxidase conjugate 500 μg MSDS    
p38 Inhibitor (SB 203580) .05 mL MSDS    

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sufficient for 96 multiwell tests

for detection of phospho-p38 alpha (pThr180 / pTyr182) and pan-p38 in human or mouse cell and tissue lysates

fore detection of phospho-p38 alpha (pThr180 / pTyr182) in human or mouse cell and tissue lysates

Safety & Documentation

Safety Information

Safety Statements (Europe) 


Certificate of Analysis

Protocols & Articles

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