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CS0750 Sigma

Caveolea/Rafts Isolation Kit

sufficient for 20 purifications

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Properties

Related Categories Cell Analysis, Cell Biology, Cell Fractionation and Organelle Isolation, Molecular Biology, Native Protein Sample Preparation,
usage   sufficient for 20 purifications
shipped in   dry ice
storage temp.   −20°C

Description

Biochem/physiol Actions

Caveolae and lipid rafts are important cholesterol and sphingolipid-rich structures that represent a subcompartment or microdomain of the plasma membrane and are implicated in cellular transport and signal transduction related events.

Application

The kit provides an easy method for obtaining fractions enriched with caveolea/rafts proteins from tissue culture cells. This method for the isolation of caveolae and rafts is based on the insolubility of these structures in ice cold non-ionic detergent Triton X-100. The kit includes all reagents for cells lysis, density gradient, and the isolation of caveolea/rafts protein enriched fraction, as well as two markers for caveolea/rafts compartments, anti-caveolin-1 and cholera toxin B subunit-peroxidase (CTB-HRP).

Price and Availability


Flow Cytometry Instruments

Ultrafree Centrifugal Filter Units

Kit component also available separately

Description

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Cholera Toxin B Subunit, Peroxidase (CTB-HRP) 50 μg SDS    
Protease inhibitor cocktail for use with mammalian cell and tissue extracts 1 mL SDS    
Anti-Caveolin-1 antibody produced in rabbit 50 μL SDS    
Triton X-100 2 mL SDS    
OptiPrep Density Gradient Medium 100 mL SDS    

Kit component only

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Lysis buffer 100 mL SDS    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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