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CS1090 Sigma

HMG-CoA Reductase Assay Kit

sufficient for 30 assays (1 mL), sufficient for 100 assays (200 μL)

Synonym: HMGR Assay Kit

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

General description

The HMG-CoA Reductase Assay Kit is an important tool for the basic research of cholesterol and other relelated metabolic pathways.

Application

The HMGR activity kit is designed for the detection of HMG-CoA reductase activity. A major function of the kit is to screen for various inhibitors and activators of the purified catalytic subunit of the enzyme, HMG-CoA reductase, which may play a crucial role in therapeutics. The assay is based on the spectrophotometric measurement of the decrease in absorbance at 340 nm, which represents the oxidation of NADPH by the catalytic subunit of HMGR in the presence of the substrate HMG-CoA.

Biochem/physiol Actions

HMGR (3-hydroxy-3-methylglutaryl-CoA reductase) is a transmembrane glycoprotein located on the endoplasmic reticulum. This enzyme catalyzes the four-electron reduction of HMG-CoA to coenzyme A (CoA) and mevalonate, which is the rate-limiting step in sterol biosynthesis. The activity of HMGR is controlled through synthesis, degradation, and phosphorylation in order to maintain the concentration of mevalonate derived products. In addition to the physiological regulation of HMGR, the human enzyme has been targeted successfully by drugs in the clinical treatment of high serum cholesterol levels. Controlling serum cholesterol levels has an important therapeutic role since hypercholesterolemia often leads to the development of atherosclerosis and subsequently to cardiovascular pathologies, which might result in myocardial infarction and stroke. It has been suggested that a disturbance of cholesterol homeostasis contributes to the development of a chronic inflammatory state.

Price and Availability


All labs need water

Kit component also available separately

Description

Product #

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β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, ≥95% (HPLC) 25 mg SDS N6505

Kit component only

Description

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Substrate Solution (HMG-CoA) 2 mL SDS    
HMG-CoA Reductase (catalytic domain) 200 μL SDS    
Assay Buffer, 5x 10 mL SDS    
Inhibitor Solution (Pravastatin) 200 μg SDS    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
For Product CS1090, HMG-CoA Reductase Assay Kit, what is the concentration of kit component Product I5909, Pravastatin?
The concentration of Pravastatin, Product I5909 in Product CS1090, HMG-CoA Reductase Assay Kit,  is 100 uM solution in assay buffer.
What is the lowest concentration that you can use Pravastatin and observe inhibition in Product CS1090, HMG-CoA Reductase Assay kit?
For Product CS1090, HMG-CoA Reductase Assay kit we do not observe any inhibition when using Pravastatin at 25 or 50 nM.  We observe inhibition of HMG-CoA Reductase with 250 nM pravastatin.
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Protocols & Articles

Articles

HMG-CoA Reductase (HMGR) Assay Kit

3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is a transmembrane glycoprotein, located on the endoplasmic reticulum.1 This enzyme catalyzes the four-electron reduction of HMG-CoA to coenzyme A and ...
BioFiles 2007, 2.7, 2.
Keywords: Cardiovascular, Catalysis, Clinical, Degradations, Enzyme activity, Inflammation, Metabolic Pathways, Oxidations, Phosphorylations, Reductions

Peer-Reviewed Papers
15

References

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