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CYP1 Sigma

CelLytic Y Plus Kit

For enzymatic yeast cell lysis

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Description

Application

The kit provides a convenient method for a highly efficient spheroplast formation and protein extraction from Saccharomyces cerevisiae, Pichia Pastoris, and Schizosaccharomyces pombe yeast strains. The procedure employs spheroplast formation by enzymatic digestion of the yeast cell wall followed by cell protein extraction (with or without detergent), while avoiding protein degradation and interference with protein immuno­reactivity and biological activity.
The cell extract is compatible with:
• Reporter gene expression assays (e.g., β-galactosidase, alkaline-phosphatase)
• Immunoassays (Western blots, immunoprecipitation)
• Affinity based purification (e.g. FLAG, glutathione S- transferase (GST) and histidine-tagged fusion proteins)
• DNA-protein interaction assay (e.g., gel-shift)
• Coomassie and silver staining
The kit is suitable for spheroplast formation and protein extraction from 10-40 grams of yeast cell pellet.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


Amplified Detection

Ultrafree Centrifugal Filter Units

Kit component only

Description

Product #

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Reaction Buffer 120 mL SDS    
1 M DTT 5 mL SDS    
Lyticase 25000 U SDS    
Extraction buffer 60 mL SDS    
Triton X-100 10 mL SDS    
Protease inhibitor cocktail 2 mL SDS    
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C
Protocols & Articles

Articles

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. Sigma® Life Science is an essent...
Keywords: Cell disruption, Gene expression, transformation

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