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CYTOCOX1 Sigma

Cytochrome c Oxidase Assay Kit

sufficient for 100 tests, soluble and membrane bound mitochondria

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Cytochrome c oxidase [EC 1.9.3.1] is located on the inner mitochondrial membrane dividing the mitochondrial matrix from the intermembrane space, and has traditionally been used as a marker for this membrane. It is also located in the cytoplasmic membrane of bacteria. Cytochrome c oxidase provides energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.

Features and Benefits

• Simple, optimized protocol - Obtain reproducible results without special training needs
• Useful for determining cytochrome c activity from any mitchondrial source - The enzyme is present in all mitochondria regardless of species
• Useful for detecting the presence of mitochondria in subcellular fraction - Save time and increase confidence in the quality of organelle preparations
• May be used in conjunction with the MITOISO1 Mitochondrial Isolation Kit - Standardized mitochondrial preparation and analysis ensures reproducibility
• Use to analyze the intactness of mitochondrial membranes
• Simple colorimetric measurement of solution

Application

The Cytochrome c Oxidase Assay Kit uses an optimized colorimetric assay based on observation of the decrease in absorbance of ferrocytochrome c measured at 550 nm, which is caused by its oxidation to ferricytochrome c by cytochrome c oxidase. This kit is suitable for the detection of mitochondrial outer membrane integrity/mitochondrial stress and for the detection of mitochondria in subcellular fractions.

Price and Availability


Detect Your Unseen Protein

Flow Cytometry Instruments

All labs need water

Kit component only

Description

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Assay Buffer 5× 25 mL SDS    
Enzyme Dilution Buffer 2× 20 mL SDS    
Cytochrome c from equine heart, ≥95% (SDS-PAGE) 50 mg SDS    
Cytochrome c Oxidase positive control 1 vial SDS    
n-Dodecyl β-D-maltoside, ≥98% (GC) 10 mg SDS    
DL-Dithiothreitol solution, 1 M .4 mL SDS    
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Organelle Isolation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, a...
Mark Frei
BioFiles v6 n5, 22–25
Keywords: Capture ELISA, Cell culture, Centrifugation, Diffusion, Direct immunofluorescence, Dot blot, Enzyme-linked immunosorbent assay, Flow cytometry, Immunoassay, Immunocytochemistry, Immunoelectrophoresis, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Indirect ELISA, Individual protein Immunoprecipitation, Microarray Analysis, Microscopy, Radioimmunoassay, Scanning electron microscopy, Western blot

Peer-Reviewed Papers
15

References

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