|Related Categories||Apoptosis and Cell Cycle, Biochemicals and Reagents, Cancer Research, Cell Analysis, Cell Biology,|
|usage||sufficient for 100 tests|
|shelf life||2 yr|
|shipped in||dry ice|
|Gene Information||human ... COX1(4512)|
Live Chat and Frequently Asked Questions are available for this Product.
The Cytochrome c Oxidase Assay Kit uses an optimized colorimetric assay based on observation of the decrease in absorbance of ferrocytochrome c measured at 550 nm, which is caused by its oxidation to ferricytochrome c by cytochrome c oxidase. This kit is suitable for the detection of mitochondrial outer membrane integrity and for the detection of mitochondria in subcellular fractions.
Cytochrome c oxidase [EC 126.96.36.199] is located on the inner mitochondrial membrane dividing the mitochondrial matrix from the intermembrane space, and has traditionally been used as a marker for this membrane. It is also located in the cytoplasmic membrane of bacteria. Cytochrome c oxidase provides energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.
• Simple, optimized protocol - Obtain reproducible results without special training needs
• Useful for determining cytochrome c activity from any mitchondrial source - The enzyme is present in all mitochondria regardless of species
• Useful for detecting the presence of mitochondria in subcellular fraction - Save time and increase confidence in the quality of organelle preparations
• May be used in conjunction with the MITOISO1 Mitochondrial Isolation Kit - Standardized mitochondrial preparation and analysis ensures reproducibility
• Use to analyze the intactness of mitochondrial membranes
• Simple colorimetric measurement of solution
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|Assay Buffer 5× 25 mL|
|Enzyme Dilution Buffer 2× 20 mL|
|Cytochrome c from equine heart, ≥95% (SDS-PAGE) 50 mg|
|Cytochrome c Oxidase positive control 1 vial|
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sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues
Certificate of Analysis
The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, a...
BioFiles v6 n5, 22–25
Keywords: Capture ELISA, Cell culture, Centrifugation, Diffusion, Direct immunofluorescence, Dot blot, Enzyme-linked immunosorbent assay, Flow cytometry, Immunoassay, Immunocytochemistry, Immunoelectrophoresis, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Indirect ELISA, Individual protein Immunoprecipitation, Microarray Analysis, Microscopy, Radioimmunoassay, Scanning electron microscopy, Western blot
iNOS initiates and sustains metabolic arrest in hypoxic lung adenocarcinoma cells: mechanism of cell survival in solid tumor core. Land, S.C., and Rae, C. Am. J. Physiol. Cell Physiol. 289(4), C918-C933, (2005)
Caveolin-1 Expression Is Essential for Proper Nonshivering Thermogenesis in Brown Adipose Tissue. Cohen, A.W., et al. Diabetes Metab. 54, 679-686, (2005)
Copper Binding by Tetrathiomolybdate Attenuates Angiogenesis and Tumor Cell Proliferation through the Inhibition of Superoxide Dismutase 1. Juarez, J.C., et al. Clin. Cancer Res. 12, 4974-4982, (2006)
Increased oxidative stress is associated with balanced increases in hepatocyte apoptosis and proliferation in glycerol-3-phosphate acyltransferase-1 deficient mice. Hammond, L.E., et al. Exp. Mol. Pathol. 82(2), 210-219, (2007)
Caspase-like proteases involvement in programmed cell death of Phaseolus coccineus suspensor. Lombardi, L., et al. Plant Sci. 172(3), 573-578, (2007)
Transcriptional regulation of pyruvate kinase and phosphoenolpyruvate carboxykinase in the adductor muscle of the oyster Crassostrea gigas during prolonged hypoxia. Le Moullac, G., et al. J. Exp. Zool. 307A(7), 371-382, (2007)
Effect of pesticides on cell survival in liver and brain rat tissues. Astiz, M., et al. Ecotox. Environ. Safety 72(7), 2025-2032, (2009)
Electron transport chain dysfunction in neonatal pressure-overload hypertrophy precedes cardiomyocyte apoptosis independent of oxidative stress. Griffiths, E.R., et al. J. Thorac. Cardiovasc. Surg. 139(6), 1609-1617, (2010)
Naturally occurring R225W mutation of the gene encoding AMP-activated protein kinase (AMPK)γ3 results in increased oxidative capacity and glucose uptake in human primary myotubes. Crawford, S.A., et al. Diabetologia 53(9), 1986-1997, (2010)
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|MITOISO1||Mitochondria Isolation Kit, sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues|
|MITOISO2||Mitochondria Isolation Kit, sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells|
|MITOISO3||Yeast Mitochondria Isolation Kit, sufficient for 40 applications (using 20 OD culture preparations), isolation of an enriched mitochondrial fraction from yeast cells|
|GL0010||Golgi Isolation Kit, sufficient for 50 g (tissue)|
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