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Cytochrome c Oxidase Assay Kit

sufficient for 100 tests, soluble and membrane bound mitochondria


Frequently Asked Questions

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Biochem/physiol Actions

Cytochrome c oxidase [EC] is located on the inner mitochondrial membrane dividing the mitochondrial matrix from the intermembrane space, and has traditionally been used as a marker for this membrane. It is also located in the cytoplasmic membrane of bacteria. Cytochrome c oxidase provides energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.

Features and Benefits

• Simple, optimized protocol - Obtain reproducible results without special training needs
• Useful for determining cytochrome c activity from any mitchondrial source - The enzyme is present in all mitochondria regardless of species
• Useful for detecting the presence of mitochondria in subcellular fraction - Save time and increase confidence in the quality of organelle preparations
• May be used in conjunction with the MITOISO1 Mitochondrial Isolation Kit - Standardized mitochondrial preparation and analysis ensures reproducibility
• Use to analyze the intactness of mitochondrial membranes
• Simple colorimetric measurement of solution


The Cytochrome c Oxidase Assay Kit uses an optimized colorimetric assay based on observation of the decrease in absorbance of ferrocytochrome c measured at 550 nm, which is caused by its oxidation to ferricytochrome c by cytochrome c oxidase.1 This kit is suitable for the detection of mitochondrial outer membrane integrity/mitochondrial stress and for the detection of mitochondria in subcellular fractions.2

Price and Availability

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Metabolomic Pathways Chart

Kit component only


Product #

Add to Cart

Assay Buffer 5× 25 mL MSDS    
Enzyme Dilution Buffer 2× 20 mL MSDS    
Cytochrome c from equine heart, ≥95% (SDS-PAGE) 50 mg MSDS    
Cytochrome c Oxidase positive control 1 vial MSDS    
n-Dodecyl β-D-maltoside, ≥98% (GC) 10 mg MSDS    
DL-Dithiothreitol solution, 1 M .4 mL MSDS    

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sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

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