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D0422 Sigma

Dulbecco’s Modified Eagle’s Medium - high glucose

With 4500 mg/L glucose and sodium bicarbonate, without L-methionine, L-cystine and L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym: DME, DMEM

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Properties

Related Categories Cell Biology, Cell Culture, Classic Media and Salts, Classical Media, Culture,
Quality Level   PREMIUM
sterility   sterile-filtered
form   liquid
impurities   endotoxin, tested
suitability   suitable for cell culture
components   glucose: yes
L-glutamine: no
phenol red: yes
storage temp.   2-8°C

Description

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Reconstitution

Supplement with 0.584 g/L L-glutamine.

Protocols & Applications

Cryostor Cryopreservation Video Protocol

Price and Availability


CellASIC® ONIX2 System

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Dulbecco's Modified Eagle's Medium - DME, DMEM

Many modifications of Eagle's Medium have been developed since the original formulation appeared in the literature. Among the most widely used of these modifications is Dulbecco's Modified Eagle's me...
Keywords: Cell culture, Substitutions, Vitamins

Peer-Reviewed Papers
15

References

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G7513 L-Glutamine solution, 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture

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