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D0426 Sigma

SIGMAFAST DAB with Metal Enhancer


Synonym: 3,3′-Diaminobenzidine Peroxidase Substrate, DAB Peroxidase Substrate



Frequently Asked Questions

Frequently Asked Questions are available for this Product.


SIGMA FAST DAB (3,3′-Diaminobenzidine tetrahydrochloride) with Metal Enhancer Tablets have been developed for use in immunohistology as a precipitating substrate for the localization of peroxidase activity. The DAB reaction is enhanced by the addition of CoCl2 and produces a more intense stain which is resistant to alcohol.

General description

SIGMA FAST DAB with Metal Enhancer Tablets require no additional buffers or steps to prepare an active substrate solution for detection of peroxidase activity. Commom use is in immunohistology as a precipitating substrate.


Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB with a metal enhancer.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

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Signal word 
NONH for all modes of transport
WGK Germany 

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What is the metal enhancer in Product D0426, SIGMAFAST™ DAB with Metal Enhancer, and what is the purpose of it?
The metal enhancer is cobalt chloride (CoCl2). Cobalt chloride produces a more intense stain which enhances the sensitivity of the assay and allows for visualization of the staining when a standard DAB chromogen would fail to demonstrate the staining.
What is a tablet set for Product D0426, SIGMAFAST™ DAB with Metal Enhancer?
This product contains two substrate tablets per tablet set; there is one DAB/cobalt tablet and one urea/hydrogen peroxide tablet. Both tablets must be used in the assay. More details may be found on the product data sheet at the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/3/d0426dat.Par.0001.File.tmp/d0426dat.pdf
How can I block endogenous peroxidase activity when using Product D0426, SIGMAFAST™ DAB with Metal Enhancer, in immunohistochemistry?
Endogenous peroxidase activity may be blocked by flooding the slide with a solution of 4 parts methanol and 1 part 3% hydrogen peroxide.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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