• USA Home
  • D0426 - SIGMAFAST DAB with Metal Enhancer

EMAIL THIS PAGE TO A FRIEND
D0426 Sigma

SIGMAFAST DAB with Metal Enhancer

tablet

Synonym: 3,3′-Diaminobenzidine Peroxidase Substrate, DAB Peroxidase Substrate

Purchase

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

SIGMA FAST DAB (3,3′-Diaminobenzidine tetrahydrochloride) with Metal Enhancer Tablets have been developed for use in immunohistology as a precipitating substrate for the localization of peroxidase activity. The DAB reaction is enhanced by the addition of CoCl2 and produces a more intense stain which is resistant to alcohol.

General description

SIGMA FAST DAB with Metal Enhancer Tablets require no additional buffers or steps to prepare an active substrate solution for detection of peroxidase activity. Commom use is in immunohistology as a precipitating substrate.

Reconstitution

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB with a metal enhancer.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


Antibody Explorer

Durapore Membrane Filters

SNAP and Go!
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Applications, Enzyme-linked immunosorbent assay, Formulations, Immunohistochemistry, Western blot

Related Content

Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Immunohistochemistry Procedures

Immunohistochemical staining is a valuable tool for detecting specific antigens in tissues. In order to perform the standard staining procedure, first the tissue section has to be deparaffinized and ...
Keywords: Digestions, Immunohistochemistry, Solvents

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?