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D0428 Sigma

1 kb DNA Ladder

for DNA electrophoresis

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Properties

Related Categories Core Bioreagents, DNA Ladder Markers, Life Science Reagents for DNA/RNA Electrophoresis, Markers and Ladders, Molecular Biology,
form   liquid
suitability   suitable for electrophoresis (DNA)
storage temp.   −20°C

Description

Components

Sigma’s 1kb Ladder is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 0.75%.

Application

1 kb DNA Ladder has been used as a molecular marker to determine the molecular weight and size of double stranded DNA during gel electrophoresis.

General description

Sigma’s 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. In nucleic acid gel electrophoresis, five μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting. All DNA markers can be stained with ethidium bromide, SYBR® Green, and Nancy-520.

Legal Information

SYBR is a registered trademark of Life Technologies

Price and Availability

Safety & Documentation
Protocols & Articles

Articles

1 kb DNA Ladder

This DNA 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb.
Keywords: DNA electrophoresis, Electrophoresis

Introduction to Markers and Ladders

Sigma-Aldrich offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments ...
Keywords: Digestions, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Markers and Ladders Selection Guide

DNA and RNA size markers contain a mixture of DNA (or RNA) fragments of known length, making them suitable for estimating the fragment length of concurrently run samples. They stain well with ethidiu...
Keywords: AGE, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Peer-Reviewed Papers
15

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D5042 123 bp DNA Ladder, for DNA electrophoresis
G2526 Gel Loading Buffer, for NA electrophoresis
D3937 DirectLoad 1 kb DNA Ladder, ready-to-use marker for DNA electrophoresis
S7025 50 bp DNA Step Ladder, for DNA electrophoresis
01494 Nancy-520, suitable for RT-PCR, ≥97.0% (HPCE)
S9430 SYBR® Green I nucleic acid gel stain, 10,000 × in DMSO
E1510 Ethidium bromide solution, BioReagent, for molecular biology, 10 mg/mL in H2O

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