D0550 Sigma

3,5-Dinitrosalicylic acid

used in colorimetric determination of reducing sugars

Synonym: 3,5-Dinitro-2-hydroxybenzoic acid, DNS


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assay   ≥98% (HPLC and titration)
form   powder
mp   168-172 °C(lit.)
solubility   H2O: soluble50 mg/mL



10, 25, 100, 500 g in glass bottle


Used in colorimetric determination of reducing sugars1,2 and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.3,4

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