• USA Home
  • D0550 - 3,5-Dinitrosalicylic acid

EMAIL THIS PAGE TO A FRIEND
D0550 Sigma

3,5-Dinitrosalicylic acid

used in colorimetric determination of reducing sugars

Synonym: 3,5-Dinitro-2-hydroxybenzoic acid, DNS

Purchase

Properties

Related Categories Cell Biology, D, Hematology and Histology, Stains & Dyes, A to Z, Stains and Dyes More...
InChI Key   LWFUFLREGJMOIZ-UHFFFAOYSA-N
assay   ≥98% (HPLC and titration)
form   powder
mp   168-172 °C(lit.)
solubility   H2O: 50 mg/mL

Description

Packaging

10, 25, 100, 500 g in glass bottle

Application

3,5-Dinitrosalicylic acid (DNS) has been used to stop the reaction in in vitro α-amylase inhibition study. It has also been used for the quantitation of enzymatically released reducing sugars.

Biochem/physiol Actions

3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.

Price and Availability


Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
VO2965000
Protocols & Articles

Protocols

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

The spectrophotometric stop reaction determination (A540, Light path = 1 cm) is based on the following reaction:

Enzymatic Assay of β-AMYLASE (EC 3.2.1.2)

3.2. Unit Definition – One unit will liberate 1.0 mg of maltose from starch in 3 minutes at pH 4.8 at 20°C.
Keywords: Enzyme activity, Enzymology

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?