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D0550 Sigma

3,5-Dinitrosalicylic acid

used in colorimetric determination of reducing sugars

Synonym: 3,5-Dinitro-2-hydroxybenzoic acid, DNS



Related Categories Cell Biology, D, Hematology and Histology, Stains & Dyes, A to Z, Stains and Dyes More...
assay   ≥98% (HPLC and titration)
form   powder
mp   168-172 °C(lit.)
solubility   H2O: soluble50 mg/mL



10, 25, 100, 500 g in glass bottle


Used in colorimetric determination of reducing sugars1,2 and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.3,4

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GHS07  GHS07
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Protocols & Articles


Enzymatic Assay of α-AMYLASE (EC

1. OBJECTIVE To standardize a procedure for determining the enzymatic activity of α-Amylase.
Keywords: Enzyme activity, Enzymology

Enzymatic Assay of β-AMYLASE (EC

1. OBJECTIVE To standardize a procedure for determining the enzymatic activity of β-Amylase.
Keywords: Enzyme activity, Enzymology

Peer-Reviewed Papers


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1. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Miller, G.L. Anal. Chem. 31, 426-8, (1959)

2. Reducing value methods for maltodextrins: I. Chain-length dependence of alkaline 3,5-dinitrosalicylate and chain-length independence of alkaline copper. Robyt, J.F. and Whelan, W.J. Anal. Biochem. 45, 510-6, (1972)


3. Comparison of the 3,5-dinitrosalicylicacid and Nelson-Somogyi methods of assaying for reducing sugars and determining cellulase activity. Breuil, C. and Saddler, J.N. Enzyme Microb. Technol. 7, 327-32, (1985)

4. Comparison of two methods for assaying reducing sugars in the determination of carbohydrase activities. Gusakov, A.V., et al. Int. J. Antimicrob. Agents, e283658, (2011)

Suggested improvements to the standard filter paper assay used to measure cellulase activity. Coward-Kelly G, Aiello-Mazzari C, Kim S, et al. Biotechnol. Bioeng. 82(6), 745-9, (2003)


Yellow stained skin from dinitro-salicylic acid. Fregert S and Trulson L Contact Dermatitis 6(5), 362, (1980)


[Photometric detection of 3,5-dinitrosalicylic acid in the air]. Kovalev AF Gig. Sanit. (9), 87, (1991)


[Adsorption of p-nitrophenol by nanosized titanium dioxide surface modified with 3,5-dinitrosalicylic acid]. Zheng FY, Qian SH, Li SX, et al. Huan Jing Ke Xue 27(6), 1140-3, (2006)


Synthesis, spectroscopic investigations, antimicrobial and DNA binding studies of a new charge transfer complex of o-phenylenediamine with 3,5-dinitrosalicylic acid. Khan IM, Ahmad A, and Ullah MF Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 102, 82-7, (2013)


A high-throughput screening system for the evaluation of biomass-hydrolyzing glycoside hydrolases. Song L, Laguerre S, Dumon C, et al. Bioresour. Technol. 101(21), 8237-43, (2010)


Optical spectroscopy of europium 3,5-dinitrosalicylates-Intense red luminophores. Zhuravlev K, Tsaryuk V, Kudryashova V, et al. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 72(5), 1020-5, (2009)


Efficient synthesis of glycosylated phenazine natural products and analogs with DISAL (methyl 3,5-dinitrosalicylate) glycosyl donors. Laursen JB, Petersen L, Jensen KJ, et al. Org. Biomol. Chem. 1(18), 3147-53, (2003)


Crystallographic characterization of the first reported crystalline form of the potent hallucinogen (R)-2-amino-1-(8-bromobenzo[1,2-b;5,4-b']difuran-4-yl)propane or 'bromodragonfly': the 1:1 anhydrous proton-transfer compound with 3,5-dinitrosalicylic acid. Smith G, Cotton MS, Wermuth UD, et al. Acta Crystallogr. C 66(Pt 5), o252-5, (2010)


Control of yeast neutral trehalase by distinct polyphosphates and ribonucleic acid. App H and Holzer H Z. Lebensm. Unters. Forsch. 181(4), 276-82, (1985)


Guest-controlling effects on ER behaviors of beta-cyclodextrin polymer. Gao ZW and Zhao XP J. Colloid. Interface Sci. 289(1), 56-62, (2005)


Optical methods for monitoring temperature in spectrophotometric analysers. O'Leary TD, Badenoch JL, and Bais R Ann. Clin. Biochem. 20(Pt 3), 153-6, (1983)


Beil. 10,IV,270

Aldrich MSDS 1, 817:A / Corp MSDS 1 (1), 1442:A / FT-IR 2 (2), 2717:C / FT-IR 1 (2), 234:A / FT-NMR 1 (2), 1152:B / IR-Spectra (3), 982:B / IR-Spectra (2), 860:G / NMR-Reference 2 (2), 230:D / RegBook 1 (2), 1823:D / Sigma FT-IR 1 (2), 519:D / Structure Index 1, 292:E:1

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