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D0550 Sigma

3,5-Dinitrosalicylic acid

used in colorimetric determination of reducing sugars

Synonym: 3,5-Dinitro-2-hydroxybenzoic acid, DNS



Related Categories Cell Biology, D, Hematology and Histology, Stains & Dyes, A to Z, Stains and Dyes More...
assay   ≥98% (HPLC and titration)
form   powder
mp   168-172 °C(lit.)
solubility   H2O: soluble50 mg/mL



10, 25, 100, 500 g in glass bottle


Used in colorimetric determination of reducing sugars1,2 and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.3,4

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GHS07  GHS07
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Protocols & Articles


Enzymatic Assay of α-AMYLASE (EC

1. OBJECTIVE To standardize a procedure for determining the enzymatic activity of α-Amylase.
Keywords: Enzyme activity, Enzymology

Enzymatic Assay of β-AMYLASE (EC

1. OBJECTIVE To standardize a procedure for determining the enzymatic activity of β-Amylase.
Keywords: Enzyme activity, Enzymology

Peer-Reviewed Papers


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1. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Miller, G.L. Anal. Chem. 31, 426-8, (1959)

2. Reducing value methods for maltodextrins: I. Chain-length dependence of alkaline 3,5-dinitrosalicylate and chain-length independence of alkaline copper. Robyt, J.F. and Whelan, W.J. Anal. Biochem. 45, 510-6, (1972)


3. Comparison of the 3,5-dinitrosalicylicacid and Nelson-Somogyi methods of assaying for reducing sugars and determining cellulase activity. Breuil, C. and Saddler, J.N. Enzyme Microb. Technol. 7, 327-32, (1985)

4. Comparison of two methods for assaying reducing sugars in the determination of carbohydrase activities. Gusakov, A.V., et al. Int. J. Antimicrob. Agents, e283658, (2011)

Size and molecular flexibility of sugars determine the storage stability of freeze-dried proteins. Tonnis WF, Mensink MA, de Jager A, et al. Mol. Pharm. 12(3), 684-94, (2015)


A rosemary extract rich in carnosic acid selectively modulates caecum microbiota and inhibits ?-glucosidase activity, altering fiber and short chain fatty acids fecal excretion in lean and obese female rats. Romo-Vaquero M, Selma MV, Larrosa M, et al. PLoS ONE 9(4), e94687, (2014)


Enhancing of sugar cane bagasse hydrolysis by Annulohypoxylon stygium glycohydrolases. Robl D, Costa Pdos S, Büchli F, et al. Bioresour. Technol. 177, 247-54, (2014)


Production of inulinase, fructosyltransferase and sucrase from fungi on low-value inulin-rich substrates and their use in generation of fructose and fructo-oligosaccharides. Rawat HK, Ganaie MA, Kango N, et al. Antonie van Leeuwenhoek 107(3), 799-811, (2015)


Effects of genetic variation and growing condition of prairie cordgrass on feedstock composition and ethanol yield. Kim SM, Guo J, Kwak S, et al. Bioresour. Technol. 183, 70-7, (2015)


Derivatization method for sensitive determination of 3-hydroxybenzo[a]pyrene in human urine by liquid chromatography-electrospray tandem mass spectrometry. Luo K, Gao Q, Hu J, et al. J. Chromatogr. A. 1379, 51-5, (2015)


Treatment of murine mast cells with IgE? and protein L enhances apoptotic cell death induced by IL-3 withdrawal. Nunomura S, Okayama Y, Terui T, et al. Biochem. Biophys. Res. Commun. 456(2), 700-5, (2014)


Lack of association of vitamin D receptor gene polymorphisms/haplotypes in Sjögren's syndrome. Zilahi E, Chen JQ, Papp G, et al. Clin. Rheumatol. 34(2), 247-53, (2015)


[Photometric detection of 3,5-dinitrosalicylic acid in the air]. Kovalev AF Gig. Sanit. (9), 87, (1991)


[Adsorption of p-nitrophenol by nanosized titanium dioxide surface modified with 3,5-dinitrosalicylic acid]. Zheng FY, Qian SH, Li SX, et al. Huan Jing Ke Xue 27(6), 1140-3, (2006)


Efficient synthesis of glycosylated phenazine natural products and analogs with DISAL (methyl 3,5-dinitrosalicylate) glycosyl donors. Laursen JB, Petersen L, Jensen KJ, et al. Org. Biomol. Chem. 1(18), 3147-53, (2003)


Yellow stained skin from dinitro-salicylic acid. Fregert S and Trulson L Contact Dermatitis 6(5), 362, (1980)


Synthesis, spectroscopic investigations, antimicrobial and DNA binding studies of a new charge transfer complex of o-phenylenediamine with 3,5-dinitrosalicylic acid. Khan IM, Ahmad A, and Ullah MF Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 102, 82-7, (2013)


Suggested improvements to the standard filter paper assay used to measure cellulase activity. Coward-Kelly G, Aiello-Mazzari C, Kim S, et al. Biotechnol. Bioeng. 82(6), 745-9, (2003)


A high-throughput screening system for the evaluation of biomass-hydrolyzing glycoside hydrolases. Song L, Laguerre S, Dumon C, et al. Bioresour. Technol. 101(21), 8237-43, (2010)


Control of yeast neutral trehalase by distinct polyphosphates and ribonucleic acid. App H and Holzer H Z. Lebensm. Unters. Forsch. 181(4), 276-82, (1985)


Optical methods for monitoring temperature in spectrophotometric analysers. O'Leary TD, Badenoch JL, and Bais R Ann. Clin. Biochem. 20(Pt 3), 153-6, (1983)


Optical spectroscopy of europium 3,5-dinitrosalicylates-Intense red luminophores. Zhuravlev K, Tsaryuk V, Kudryashova V, et al. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 72(5), 1020-5, (2009)


Guest-controlling effects on ER behaviors of beta-cyclodextrin polymer. Gao ZW and Zhao XP J. Colloid. Interface Sci. 289(1), 56-62, (2005)


Crystallographic characterization of the first reported crystalline form of the potent hallucinogen (R)-2-amino-1-(8-bromobenzo[1,2-b;5,4-b']difuran-4-yl)propane or 'bromodragonfly': the 1:1 anhydrous proton-transfer compound with 3,5-dinitrosalicylic acid. Smith G, Cotton MS, Wermuth UD, et al. Acta Crystallogr. C 66(Pt 5), o252-5, (2010)


Beil. 10,IV,270

Aldrich MSDS 1, 817:A / Corp MSDS 1 (1), 1442:A / FT-IR 2 (2), 2717:C / FT-IR 1 (2), 234:A / FT-NMR 1 (2), 1152:B / IR-Spectra (3), 982:B / IR-Spectra (2), 860:G / NMR-Reference 2 (2), 230:D / RegBook 1 (2), 1823:D / Sigma FT-IR 1 (2), 519:D / Structure Index 1, 292:E:1

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