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D0672 Sigma

φX174 DNA Marker Hae III Digest

for DNA electrophoresis

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Properties

Related Categories DNA Digest Markers, Markers and Ladders, Molecular Biology, Nucleic Acid Electrophoresis
form   liquid
usage   200 uses (0.1 mg size)
  40 uses (20 μg size)
suitability   suitable for electrophoresis (DNA)
storage temp.   −20°C

Description

Components

Sigma’s Phi X174 DNA Marker Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

General description

Sigma’s Phi X174 DNA Marker Hae III Digest contains 11 fragments from 72 − 1,353 bp. Five ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Preparation Note

φX174RF-I DNA was digested with Hae III to completion, then purified by phenol extraction.

Price and Availability

Protocols & Articles

Articles

Introduction to Markers and Ladders

Sigma-Aldrich offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments ...
Keywords: Digestions, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Markers and Ladders Selection Guide

DNA and RNA size markers contain a mixture of DNA (or RNA) fragments of known length, making them suitable for estimating the fragment length of concurrently run samples. They stain well with ethidiu...
Keywords: AGE, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

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