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D0690 Sigma

DNA Gyrase from Escherichia coli

aqueous glycerol solution

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Properties

Related Categories 5.99.x.x Others, 5.x.x.x Isomerases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
form   aqueous glycerol solution
mol wt   mol wt ~374 kDa
concentration   ≥2 unit/μL
shipped in   dry ice
storage temp.   −70°C
Gene Information   Escherichia coli K12 ... gyrA(946614), gyrB(948211)

Description

Biochem/physiol Actions

Can be used to supercoil plasmids.

DNA gyrase is supplied as an A2B2 holoenzyme. The molecular mass of subunit A is 97 kDa and that of subunit B is 90 kDa. It catalyzes the ATP-dependent introduction of negative supercoils into relaxed DNA. DNA gyrase has been successfully converted into a type II topoisomerase by mutagenesis experiments.

Other Notes

Solution in 50% Glycerol containing Tris buffer, DTT and EDTA, 5X Reaction buffer included.

Unit Definition

One unit of gyrase activity will supercoil 0.5 micrograms of pBR-322 DNA in 30 minutes at 37 °C.

Application

DNA gyrase from Escherichia coli has been used in a study to investigate a comparative proteomic approach to better define Deinococcus nucleoid specificities. DNA gyrase from Escherichia coli has also been used in a study to investigate the role of the DnaK-ClpB bichaperone system in DNA gyrase reactivation.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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