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D0690 Sigma

DNA Gyrase from Escherichia coli

aqueous glycerol solution



Related Categories 5.99.x.x Others, 5.x.x.x Isomerases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
form   aqueous glycerol solution
mol wt   mol wt ~374 kDa
concentration   ≥2 unit/μL
shipped in   dry ice
storage temp.   −70°C
Gene Information   Escherichia coli K12 ... gyrA(946614), gyrB(948211)


Biochem/physiol Actions

Can be used to supercoil plasmids.

DNA gyrase is supplied as an A2B2 holoenzyme. The molecular mass of subunit A is 97 kDa and that of subunit B is 90 kDa. It catalyzes the ATP-dependent introduction of negative supercoils into relaxed DNA. DNA gyrase has been successfully converted into a type II topoisomerase by mutagenesis experiments.

Other Notes

Solution in 50% Glycerol containing Tris buffer, DTT and EDTA, 5X Reaction buffer included.

Unit Definition

One unit of gyrase activity will supercoil 0.5 micrograms of pBR-322 DNA in 30 minutes at 37 °C.


DNA gyrase from Escherichia coli has been used in a study to investigate a comparative proteomic approach to better define Deinococcus nucleoid specificities. DNA gyrase from Escherichia coli has also been used in a study to investigate the role of the DnaK-ClpB bichaperone system in DNA gyrase reactivation.

Price and Availability

Biomedical Applications
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers


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