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D0879 Sigma

Diisopropylfluorophosphate

Synonym: DFP, DIFP, Diisopropyl phosphorofluoridate, Phosphoric acid diisopropyl ester fluoride

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Properties

Related Categories A to C, Acetylcholinesterase, Analytical and Industrial Enzymes, Apoptosis Enzymes, Application Index,
vapor pressure   0.58 mmHg ( 20 °C)
InChI Key   MUCZHBLJLSDCSD-UHFFFAOYSA-N
refractive index   n20/D 1.385(lit.)
bp   62 °C/9 mmHg(lit.)
mp   −82 °C(lit.)
solubility   anhydrous isopropanol: soluble0.1-0.5 M (stable for months if stored at -70 °C)
  H2O: soluble1.5% at 25 °C (very unstable (at pH 7.5, half-life = 1 hr); decomposed by alkali.)
density   1.06 g/mL at 25 °C(lit.)
storage temp.   2-8°C
Gene Information   human ... PTGS1(5742), PTGS2(5743)

Description

Analysis Note

Typically used at a concentration of 0.10 mM. A safer alternative inhibitor for serine protease is phenylmethylsulfonyl fluoride (PMSF).

Other Notes

Stored properly at 2-8°C, in an unopened bottle, DIFP should be stable for a minimum of two years. DIFP
is unstable when exposed to moisture. DIFP will develop a dark yellow color upon decomposition.

Biochem/physiol Actions

Potent inhibitor of serine proteases such as trypsin and chymotrypsin, and of acetylcholinesterase; also inhibits cathepsin G, cholinesterase, coagulation factor Xa, leucocyte elastase, pancreatic elastase, tissue kallikrein, plasmin, subtilisin, and thrombin. Inhibition of acetylcholinesterase makes this compound especially toxic. Inhibits apoptosis induced by ricin and bacterial toxins.

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Safety & Documentation

Safety Information

Symbol 
GHS06  GHS06
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3382 6.1 / PGI
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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