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D1033 Sigma

Monoclonal Anti-Desmin antibody produced in mouse

clone DE-U-10, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cardiac Stem Cells, Antibodies for Cell Biology, Antibodies for Mesenchymal Stem Cells,
species reactivity   goat, mouse, bovine, rat, hamster, rabbit, chicken, viper, feline, lizard, sheep, human
application(s)   indirect immunofluorescence: 1:40 using formalin-fixed, paraffin-embedded sections of human tissue
  microarray: suitable
  western blot: 1:200 using chicken gizzard extract
clone   DE-U-10, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 50-55 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Des(13346)
rat ... Des(64362)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

desmin from pig stomach

General description

Desmin is the protein subunit of muscle-type intermediate filaments and is found predominantly in skeletal, cardiac, and smooth muscle.

Specificity

The antibody localizes desmin in cultured cells and tissue sections from several species and in tumors derived from muscle tissue (leiomyomas, rhabdomyosarcomas).

Application

Monoclonal Anti-Desmin antibody produced in mouse is suitable for indirect immunofluorescence at a working dilution of 1:20 using formalin-fixed, paraffin-embedded sections of human tissue, microarray and western blotting. It may be used for immunocytochemical localization of intermediate filaments of the desmin group in all types of muscle cells and to localize desmin at the periphery of z-discs. It specifically stains desmin when used in immunoblotting assays. It may be used for labelling desmin in tumors derived from muscle tissue (leiomyomas and rhabdomyosarcomas). It was used for immunohistochemical staining of cardiac cells in a study to understand cardiac conduction through engineered tissue. It was used as a primary antibody for the detection of expression of desmin in cultured cells to study the effect of mutation in desmin gene.

Features and Benefits

Antibody Bioguarantee
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Biochem/physiol Actions

Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.

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