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D1152 Sigma

Dulbecco’s Modified Eagle’s Medium - high glucose

HEPES Modification, With 4500 mg/L glucose, L-glutamine, and 25 mM HEPES, without sodium bicarbonate and pyruvate, powder, suitable for cell culture

Synonym: DME, DMEM



Related Categories Cell Culture, Classic Media and Salts, Dulbecco’s Media, Dulbecco’s Modified Eagle’s Medium (DMEM), Powder Media More...
Quality Level   GMP
form   powder
suitability   suitable for cell culture
components   HEPES: 25 mM
glucose: high
L-glutamine: yes
NaHCO3: no
phenol red: yes
sodium pyruvate: no
storage temp.   2-8°C



Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".


Formulated to contain 17.3 grams of powder per liter of medium.


Supplement with 3.7 g/L sodium bicarbonate.

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