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D1190 Sigma

DT Diaphorase from rat

~90% (SDS-PAGE), lyophilized powder, recombinant, expressed in E. coli

Synonym: DTD, NQO1, Quinone reductase



Related Categories 1.6.x.x Acting on NAD or NADP, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates More...
recombinant   expressed in E. coli
assay   ~90% (SDS-PAGE)
form   lyophilized powder
mol wt   monomer mol wt 31000
storage temp.   2-8°C
Gene Information   rat ... Nqo1(24314)


Biochem/physiol Actions

Shown to activate quinone based anti-tumor agents in vivo. Suitable for conjugation to carrier molecules.

DT Diaphorase is a flavoenzyme that catalyzes the oxidation of reduced di- and triphosphopyridine nucleotides. It contains one mole of FAD per mole of enzyme. The enzyme found in rat liver catalyzes the oxidation of NADH and NADPH by various dyes and quinones. The molecular weight is found to be approximately 48,000 Da. The pH optimum of the enzyme purified from rat liver is found to be 5.0. It is a cytosolic enzyme that catalyzes the two-electron reduction of various quinones. It catalyzes the conversion of vitamin K to vitamin K hydroquinone for utilization in the post-translational γ-glutamyl carboxylation reactions. These reactions are necessary for several proteins involved in blood coagulation.

Other Notes

Catalytic activity is different from human source DT diaphorase

Unit Definition

One unit will reduce 1.0 μmole cytochrome C per min/mg in the presence of menadione substrate at 37 °C.

Physical form

Lyophilized powder containing approx. 5% protein balance lactose and PBS buffer salts


DT Diaphorase from rat has been used in a study to investigate the two-electron reduction of quinones by rat liver. DT Diaphorase from rat has also been used in a study to investigate colon cancer chemopreventive efficacy of silibinin through perturbation of xenobiotic metabolizing enzymes.

Price and Availability

Biomedical Applications
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers


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Add to Cart

D1315 DT Diaphorase (NQO1) human, lyophilized powder, recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
HPA007308 Anti-NQO1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
N5288 Anti-NQO1 (C-terminal) antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

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