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D1190 Sigma

DT Diaphorase from rat

~90% (SDS-PAGE), lyophilized powder, recombinant, expressed in E. coli

Synonym: DTD, NQO1, Quinone reductase

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Properties

Related Categories 1.6.x.x Acting on NAD or NADP, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates More...
recombinant   expressed in E. coli
assay   ~90% (SDS-PAGE)
form   lyophilized powder
mol wt   monomer mol wt 31000
storage temp.   2-8°C
Gene Information   rat ... Nqo1(24314)

Description

Biochem/physiol Actions

Shown to activate quinone based anti-tumor agents in vivo. Suitable for conjugation to carrier molecules.

DT Diaphorase is a flavoenzyme that catalyzes the oxidation of reduced di- and triphosphopyridine nucleotides. It contains one mole of FAD per mole of enzyme. The enzyme found in rat liver catalyzes the oxidation of NADH and NADPH by various dyes and quinones. The molecular weight is found to be approximately 48,000 Da. The pH optimum of the enzyme purified from rat liver is found to be 5.0. It is a cytosolic enzyme that catalyzes the two-electron reduction of various quinones. It catalyzes the conversion of vitamin K to vitamin K hydroquinone for utilization in the post-translational γ-glutamyl carboxylation reactions. These reactions are necessary for several proteins involved in blood coagulation.

Other Notes

Catalytic activity is different from human source DT diaphorase

Unit Definition

One unit will reduce 1.0 μmole cytochrome C per min/mg in the presence of menadione substrate at 37 °C.

Physical form

Lyophilized powder containing approx. 5% protein balance lactose and PBS buffer salts

Application

DT Diaphorase from rat has been used in a study to investigate the two-electron reduction of quinones by rat liver. DT Diaphorase from rat has also been used in a study to investigate colon cancer chemopreventive efficacy of silibinin through perturbation of xenobiotic metabolizing enzymes.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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D1315 DT Diaphorase (NQO1) human, lyophilized powder, recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
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