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D1315 Sigma

DT Diaphorase (NQO1) human

lyophilized powder, recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym: DTD, NQO1, Quinone reductase

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Properties

Related Categories 1.6.x.x Acting on NAD or NADP, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates More...
recombinant   expressed in E. coli
assay   ≥90% (SDS-PAGE)
form   lyophilized powder
activity   ≥100 units/mg protein
mol wt   monomer mol wt 31000
storage temp.   2-8°C
Gene Information   human ... NQO1(1728)

Description

Biochem/physiol Actions

Shown to activate quinone based anti-tumor agents in vivo. Suitable for conjugation to carrier molecules.

DT-diaphorase, also referred to as NAD(P)H:(quinone-acceptor) oxidoreductase, is involved in the reductive activation process of several cytotoxic antitumor quinones and nitrobenzenes. It catalyzes the two-electron reduction of quinones and quinonoid compounds to hydroquinones, using either NADH or NADPH as the electron donor. The flavoenzyme contains one mole of FAD per mole of enzyme.

Unit Definition

One unit will reduce 1.0 μmole cytochrome C per min/mg in the presence of menadione substrate at 37 °C.

Application

Human DT diaphorase has been used in a study to assess the development of novel quinone phosphorodiamidate prodrugs. Human DT diaphorase has also been used to investigate its crystal structure for the development of a model for its interaction with the cytotoxic prodrug 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB1954).

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Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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