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D1439 Sigma

Anti-DRAK2 antibody produced in rabbit

~0.5 mg/mL, IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology,
species reactivity   human
application(s)   western blot: suitable
clone   polyclonal
concentration   ~0.5 mg/mL
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 45 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... STK17B(9262)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 351-365 of the C-terminal region of human DRAK2.

General description

DRAKs contain an N-terminal kinase domain and a C-terminal regulation domain. They have high sequence homology to DAP and ZIP kinases. They are localized to the nucleus.

Physical form

Solution in phosphate buffered saline, containing 0.02% sodium azide.

Application

Anti-DRAK2 antibody produced in rabbit is suitable for immunoblotting at a working concentration of 0.5-1 μg/mL (1:1000 – 1:500 dilution) using human Jurkat and Raji cell lysates.

Features and Benefits

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Biochem/physiol Actions

DAP kinase-related apoptosis-inducing protein kinase 2 (DRAK2) is a serine/threonine kinase that induces apoptosis. DRAK1 and DRAK2 show 59.7% identity and display ubiquitous expression. Both DRAK1 and DRAK2 are autophosphorylated and phosphorylate myosin light chain as an exogenous substrate.

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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