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D1501 Sigma

Deoxyribonucleic acid sodium salt from calf thymus

Type I, fibers

Synonym: DNA sodium salt from calf thymus, Thymonucleic acid sodium salt, ctDNA

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Properties

Related Categories Biochemicals and Reagents, Core Bioreagents, Nucleosides and Nucleotides, Nucleosides, Nucleotides, Oligonucleotides, Oligonucleotides, DNA, and RNA,
Quality Level   PREMIUM
type   Type I
form   fibers
color   white
storage temp.   2-8°C

Description

Packaging

1, 5 g in poly bottle

50, 100, 500 mg in poly bottle

Preparation Note

This product is extracted using a method that causes shearing yielding a highly polymerized mixture of double and single stranded DNA . However, double stranded DNA is the predominant form.

This product is prepared from calf thymus tissue of unspecified gender.

Quality

"Highly Polymerized"

Reconstitution

Solutions of DNA have been stored successfully for several months at 4 C, pH 7.5-8, in 10 mM Tris with 1 mM EDTA and without a bacteriostatic agent. At low concentrations (μg/ml) DNA tends to absorb onto the surfaces of plastic tubes. It is not recommended to store DNA in highly alkaline solutions since DNA tends to degrade at alkaline pH greater than 8.0.

This product can be dissolved at 2 mg/ml in water. To reduce further shearing of the DNA, no sonication or stirring should be used. Gentle inversion overnight at 0-4 °C is recommended to completely solubilize the DNA. The presence of 1 mM EDTA is recommended to prevent nucleases from degrading the DNA.

Physical form

fibrous preparation

Application

Calf thymus DNA (CT-DNA) is a natural DNA widely used in studies of DNA binding anticancer agents and DNA binding agents that modulate DNA structure and function. Calf thymus DNA is also used in physicochemical studies of DNA behavior in solution.

Physical properties

λmax 259 nm (100 mM phosphate buffer, pH 7.0).
DNA from calf thymus is 41.9 mole % G-C and 58.1 mole % A-T.1 An OD of 1.0 at 260 nm corresponds to ~ 50 μg of double-stranded DNA.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Enzymatic Assay of Nuclease S1, Product No. N5661

To standardize a procedure for the enzymatic assay of Nuclease S1, Sigma Product Number N5661 at Sigma-Aldrich St. Louis.

Peer-Reviewed Papers
15

References

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