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D1530 Sigma

1α,25-Dihydroxyvitamin D3

≥99% (HPLC)

Synonym: 1α,25-Dihydroxycholecalciferol, Calcitriol

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Properties

Related Categories Biochemicals and Reagents, Vitamin D, Vitamin D3, Vitamins
Quality Level   PREMIUM
InChI Key   GMRQFYUYWCNGIN-NKMMMXOESA-N
assay   ≥99% (HPLC)
storage temp.   −20°C
solubility   Do you have solubility information on this product that you would like to share?
Gene Information   human ... VDR(7421)

Description

Frequently Asked Questions

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Application

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Biochem/physiol Actions

Biologically active form of vitamin D3 in calcium absorption and deposition. 1α,25-Dihydroxyvitamin D3 has widespread effects on cellular differentiation and proliferation, and can modulate immune responsiveness, and central nervous system function. Recent studies suggest that 1α,25-dihydroxyvitamin D3 acts as a chemopreventive agent against several malignancies including cancers of the prostate and colon and shows synergy with other anticancer compounds.

Cholecalciferol is an inactive form of vitamin D3 which undergoes various levels of hydroxylation to form active vitamin D3 analogs. 1α-Hydroxyvitamin D3 (alfacalcidol is a synthetic analog that is metabolized to 1,25-dihydroxycholecalciferol, the biologically active form of vitamin D3 in calcium absorption and deposition. 1α,25-Dihydroxyvitamin D3 has widespread effects on cellular differentiation and proliferation, and can modulate immune responsiveness, and central nervous system function. Recent studies suggest that 1α,25-dihydroxyvitamin D3 acts as a chemopreventive agent against several malignancies including cancers of the prostate and colon and shows synergy with other anticancer compounds.

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Safety & Documentation

Safety Information

Symbol 
GHS06  GHS06
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2811 6.1 / PGI
WGK Germany 
3
RTECS 
FZ4645000

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
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A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
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There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
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Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
How can I dissolve Product D1530, 1α,25-Dihydroxyvitamin D3, for cell culture use?
1α,25-Dihydroxyvitamin D3 is soluble in serveral organic solvents, including methanol, ethanol, ethyl acetate, and tetrahydrofuran. For cell culture use, a 10 µM stock solution can be prepared and diluted to the working concentration in cell culture media.
What is the best way to store stock solutions of Product D1530, 1α,25-Dihydroxyvitamin D3?
1α,25-Dihydroxyvitamin D3 is both air and light sensitive, so should be stored under inert gas at either -20 or -70°C.  The length of time a stock solution can be stored depends on how carefully air is excluded from the solution. If care is taken to exclude air, stock solutions may be stable indefinitely at -20°C.
I can't see anything in the vial. How should Product D1530, 1α,25-Dihydroxyvitamin D3, look?
D1530 is packaged by dispensing a solution into the vials, then drying them under inert gas. The product will be a thin film in the bottom of the vial, so it may appear to be empty by visual inspection.
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Protocols & Articles

Articles

Vitamin D and Prevention of Human Disease

Vitamin D2 (ergocalciferol) is naturally synthesized from ergosterol by invertebrates, fungi, and plants in response to ultraviolet B irradiation, while vitamin D3 synthesis (cholecalciferol) is uniq...
Chloe McClanahan
BioFiles 2009, 4.6, 4.
Keywords: Absorption, Angiogenesis, Biofiles, Biological processes, Cancer, Clinical, Gene expression, Growth factors, Hormones, Hydroxylations, Ligands, Metabolites, Transcription, Type, Vitamins

Peer-Reviewed Papers
15

References

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