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D1556 Sigma

OptiPrep Density Gradient Medium

used for cell and subcellular organelle isolation

Synonym: Iodixanol solution

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Description

Frequently Asked Questions

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Application

Density gradient suitable for isolation of cells and cell organelles, virus purification, and isolation of lipoproteins and macromolecules.

Other Notes

60% (w/v) solution of iodixanol in water (sterile).

Packaging

250 mL in glass bottle

Legal Information

OptiPrep is a trademark of Axis-Shield PoC AS

Price and Availability

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Safety & Documentation

Safety Information

WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Peer-Reviewed Papers

References

Set your institution to view full text papers.

The preparation of subcellular organelles from mouse liver in self-generated gradients of iodixanol. Graham, J., Ford, T., and Rickwood, D. Anal. Biochem. 220, 367, (1994)

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Adeno-associated virus gene therapy with cholesterol 24-hydroxylase reduces the amyloid pathology before or after the onset of amyloid plaques in mouse models of Alzheimer's disease. Hudry E, Van Dam D, Kulik W, et al. Mol. Ther. 18(1), 44-53, (2010)

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Intramembrane processing by signal peptide peptidase regulates the membrane localization of hepatitis C virus core protein and viral propagation. Okamoto K, Mori Y, Komoda Y, et al. J. Virol. 82(17), 8349-61, (2008)

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Lipoprotein lipase inhibits hepatitis C virus (HCV) infection by blocking virus cell entry. Maillard P, Walic M, Meuleman P, et al. PLoS ONE 6(10), e26637, (2011)

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Activation of Src and release of intracellular calcium by phosphatidic acid during Xenopus laevis fertilization. Bates RC, Fees CP, Holland WL, et al. Dev. Biol. 386(1), 165-80, (2014)

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Direct infection and replication of naturally occurring hepatitis C virus genotypes 1, 2, 3 and 4 in normal human hepatocyte cultures. Martina Buck et al PLoS ONE 3, e2660, (2008)

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Japanese encephalitis virus replication is negatively regulated by autophagy and occurs on LC3-I- and EDEM1-containing membranes. Sharma M, Bhattacharyya S, Nain M, et al. Autophagy 10(9), 1637-51, (2014)

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Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: Differential expression of neuronal and glial protein markers. Balmiki Ray et al J. Neurosci. Methods 184, 294-302, (2010)

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Endothelin-1 induced desensitization in primary afferent neurons. Smith TP, Smith SN, and Sweitzer SM Neurosci. Lett. 582, 59-64, (2014)

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