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D1556 Sigma

OptiPrep Density Gradient Medium

used for cell and subcellular organelle isolation

Synonym: Iodixanol solution



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Density gradient suitable for isolation of cells and cell organelles, virus purification, and isolation of lipoproteins and macromolecules.

Other Notes

60% (w/v) solution of iodixanol in water (sterile).


250 mL in glass bottle

Legal Information

OptiPrep is a trademark of Axis-Shield PoC AS

Price and Availability

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Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers


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Comparative Analysis of Cesium Chloride- and Iodixanol-Based Purification of Recombinant Adeno-Associated Viral Vectors for Preclinical Applications. Strobel B, Miller FD, Rist W, et al. Hum. Gene Ther. Methods 26(4), 147-57, (2015)


The preparation of subcellular organelles from mouse liver in self-generated gradients of iodixanol. Graham, J., Ford, T., and Rickwood, D. Anal. Biochem. 220, 367, (1994)


Direct binding of retromer to human papillomavirus type 16 minor capsid protein L2 mediates endosome exit during viral infection. Popa A, Zhang W, Harrison MS, et al. PLoS Pathog. 11(2), e1004699, (2015)


Structure-based mutational analysis of several sites in the E protein: implications for understanding the entry mechanism of Japanese encephalitis virus. Liu H, Liu Y, Wang S, et al. J. Virol. 89(10), 5668-86, (2015)


A comparative study of two different assay kits for the detection of secreted alkaline phosphatase in HPV antibody neutralization assays. Kemp TJ, Matsui K, Shelton G, et al. Hum. Vaccin. Immunother. 11(2), 337-46, (2015)


Adeno-associated virus gene therapy with cholesterol 24-hydroxylase reduces the amyloid pathology before or after the onset of amyloid plaques in mouse models of Alzheimer's disease. Hudry E, Van Dam D, Kulik W, et al. Mol. Ther. 18(1), 44-53, (2010)


Intramembrane processing by signal peptide peptidase regulates the membrane localization of hepatitis C virus core protein and viral propagation. Okamoto K, Mori Y, Komoda Y, et al. J. Virol. 82(17), 8349-61, (2008)


Lipoprotein lipase inhibits hepatitis C virus (HCV) infection by blocking virus cell entry. Maillard P, Walic M, Meuleman P, et al. PLoS ONE 6(10), e26637, (2011)


Activation of Src and release of intracellular calcium by phosphatidic acid during Xenopus laevis fertilization. Bates RC, Fees CP, Holland WL, et al. Dev. Biol. 386(1), 165-80, (2014)


Direct infection and replication of naturally occurring hepatitis C virus genotypes 1, 2, 3 and 4 in normal human hepatocyte cultures. Martina Buck et al PLoS ONE 3, e2660, (2008)


Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: Differential expression of neuronal and glial protein markers. Balmiki Ray et al J. Neurosci. Methods 184, 294-302, (2010)


Japanese encephalitis virus replication is negatively regulated by autophagy and occurs on LC3-I- and EDEM1-containing membranes. Sharma M, Bhattacharyya S, Nain M, et al. Autophagy 10(9), 1637-51, (2014)


Endothelin-1 induced desensitization in primary afferent neurons. Smith TP, Smith SN, and Sweitzer SM Neurosci. Lett. 582, 59-64, (2014)


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