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  • D1667 - Monoclonal Anti-Dynein (Heavy Chain) antibody produced in mouse

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D1667 Sigma

Monoclonal Anti-Dynein (Heavy Chain) antibody produced in mouse

clone 440.4, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
species reactivity   human, chicken
application(s)   indirect ELISA: suitable
  western blot: 1:100 using a chick brain extract or Kinesin enriched rat brain extract
clone   440.4, monoclonal
antibody form   ascites fluid
isotype   IgG2a
mol wt   antigen mol wt 440 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... DNAH1(25981), DNAH2(146754), DNAH3(55567), DNAH5(1767)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

cytoplasmic dynein from chicken embryo brain.

General description

Dynein is a motor protein that regulates the movement of organelles to the ‘minus’ end of microtubules. Dyenins consist of two heavy chains and many intermediate and light chains. These motor proteins are also involved in spindle organization, retrograde transport and chromosome movement
In immunoblotting assays, monoclonal anti-dynein (Heavy Chain) is specific for cytoplasmic dynein heavy chain (440 kDa) using dynein enriched preparations, as well as in crude cell extracts of chicken brain and fibroblasts. Upon cleavage of the heavy chain into two fragments (of approximately 200 kDa by exposure to U.V. light in the presence of ATP and vanadate), it is observed that the antibody reacts exclusively with the lower molecular weight fragment. The product reacts with kinetochores in HeLa chromosomes but does not bind to chromosomal dynein in Chinese hamster ovary cell line. Moreover, the antibody product does not react with Chlamydomonas flagella dynein.

Application

Monoclonal Anti-Dynein (Heavy Chain) antibody is suitable for use in western blot (1:100 using a chick brain extract) and indirect ELISA. The product may also be used for immunoblot, dot blot and immunocytochemistry. Using immunofluorescent techniques, the staining of the spindle with the antibody is clearly visible through diffuse cytoplasmic staining in intact mitotic chicken embryo fibroblasts.

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