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  • D1689 - Anti-Deoxyribonuclease II (DNase II) antibody produced in rabbit

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D1689 Sigma

Anti-Deoxyribonuclease II (DNase II) antibody produced in rabbit

~0.5 mg/mL, affinity isolated antibody

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to DNA Fragmentation,
species reactivity   human
application(s)   microarray: suitable
  western blot: 0.5-1 μg/mL using a human spleen lysate
clone   polyclonal
concentration   ~0.5 mg/mL
antibody form   affinity isolated antibody
mol wt   antigen mol wt 32 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... DNASE2(1777)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 347-360 of human DNAse II precursor.

Application

Anti-Deoxyribonuclease II (DNase II) antibody produced in rabbit is suitable for microarray and immunoblotting at a working concentration of 0.5-1 μg/mL (1:1000 – 1:500 dilution) using a human spleen lysate. It was used at a working concentration of 0.25 μg/mL to examine the immunoreactivity of samples prepared from outer hair cells of guinea pigs’ inner ear in a study.

Features and Benefits

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Biochem/physiol Actions

DNase II digests DNA at low pH and acts as a lysosomal enzyme responsible for degrading the exogenous DNA that enters the cell. In the nucleus, it degrades the DNA during apoptosis. It causes both chromatin condensation and DNA fragmentation. It is involved in DNA fragmentation induced by lovastatin through activation of caspase-3 and DNase II activities in HL-60 cells. The gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (α) and 12 kDa (β) chains, generated by proteolytic processing.

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