• USA Home
  • D1793 - Lambda DNA Pst I Digest

EMAIL THIS PAGE TO A FRIEND
D1793 Sigma

Lambda DNA Pst I Digest

buffered aqueous solution

Purchase

Properties

grade   for molecular biology
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C

Description

Application

Pst I digested bacteria phage Lambda DNA is suitable for size determination of double-stranded DNA using DNA electrophoresis. Some of the 29 fragments are seen as heavy bands which are actually composed of doublets or triplets. The recommended agarose gel concentration is 1.0% for this marker. A sample of the marker should be diluted with gel loading buffer to an appropriate loading concentration. Typically 500 ng per well (50 ng/μl, 10 μl load) is sufficient to be seen using ethidium bromide staining.

Other Notes

Contains 29 fragments, 15–11,497 bp

Physical form

Solution in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Nancy-520 for DNA Detection and Quantitation

Nancy-520 is a fluorescent stain for double stranded DNA (dsDNA) on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is...
Monika Baeumle
BioFiles 2009, 4.1, 4.
Keywords: Catalytic combustion detector, Electrophoresis

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?