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D1806 Sigma

Taq DNA Polymerase from Thermus aquaticus

with 10× PCR reaction buffer containing MgCl2

Purchase

Properties

Related Categories 2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Application Index, Biochemicals and Reagents, Core Bioreagents,
recombinant   expressed in E. coli
form   liquid
feature   hotstart: No
concentration   5 units/μL
color   colorless
  colorless
suitability   suitable for PCR and automated sequencing reactions
Featured Industry   Agriculture
shipped in   wet ice
storage temp.   −20°C

Description

Features and Benefits

• Low per unit cost of Taq

Other Notes

Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 5′→3′ polymerase and exonuclease activity.

Packaging

Taq DNA Polymerase with 10× reaction buffer containing MgCl2

Taq DNA polymerase comes with the choice of an optimized 10× reaction buffer including MgCl2 (D1806) or a 10× reaction buffer without MgCl2 plus a separate tube of MgCl2 for titration (D4545). The latter option may be necessary to determine optimal conditions for amplification.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Unit Definition

One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Application

Taq DNA Polymerase from Thermus aquaticus is a thermostable DNA polymerase that is used for the DNA polymerase chain reaction (PCR) in order to amplify DNA sequences.

Biochem/physiol Actions

Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Articles

Improving Real-Time PCR Success

To meet increasing food supply demands of the global population, growers require seeds that can withstand the regional challenges found in their fields. In order to develop crop varieties that are mo...
Keywords: Agriculture, Amplification, Environmental, Polymerase chain reaction

Introduction & Historical Timelines - PCR Technologies Guide

The Polymerase Chain Reaction (PCR) is used in all areas of biological science research, including the clinical, forensic and diagnostic fields and the widespread adoption of the PCR technique has re...
Keywords: Amplification, Clinical, Degradations, Detection methods, Diagnostic, Diseases, Forensic, Gene expression, Genetic, Molecular biology, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Separation, Transcription

Protocols

Standard PCR protocol

Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. The enzyme is in a recombinant form, expressed in E. coli. It is able to withstand repeated heat...
Keywords: AGE, Amplification, Applications, Electrophoresis, Evaporation, Gel electrophoresis, PAGE, Polymerase chain reaction, Sequencing

dNTP Mediated Hot Start PCR Protocol

Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecti...
Keywords: AGE, Amplification, Buffers, Centrifugation, Degradations, Electrophoresis, Gel electrophoresis, Nucleic acid denaturation, Polymerase chain reaction, Purification, Size-exclusion chromatography, Transcription

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Sigma-Aldrich’s chemistry expertise can help customers develop and optimize their leaf and seed genotyping workflow to reduce cost per data point while increasing throughput rates. In addition, our g...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing, Whole genome amplification

Agriculture | Plant Breeding Workflow

Sigma-Aldrich products are aligned to the discovery, development and production phases of the plant breeding workflow, allowing you to develop and produce new crop varieties faster. With materials ma...

Enzymes & Proteins

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PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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Add to Cart

D4545 Taq DNA Polymerase from Thermus aquaticus, with 10× PCR reaction buffer without MgCl2
D4309 REDTaq® DNA Polymerase, Taq for routine PCR with inert dye, 10X buffer included

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