D1821 Sigma


≥98% (HPLC)

Synonym: 4-Amino-5-methylamino-2′,7′-difluorescein, Diaminofluorescein-FM

  • CAS Number 254109-20-1

  • Empirical Formula (Hill Notation) C21H14F2N2O5

  • Molecular Weight 412.34


Related Categories Cell Biology, Cell Signaling and Neuroscience, Nitric Oxide Detection, Nitric Oxide Metabolism, Nitric Oxide and Cell Stress More...
assay   ≥98% (HPLC)
form   powder
storage condition   desiccated
color   white to beige
solubility   DMSO: >1 mg/mL
shipped in   wet ice
storage temp.   −20°C




Biochem/physiol Actions

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.

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