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D1821 Sigma

DAF-FM

≥98% (HPLC)

Synonym: 4-Amino-5-methylamino-2′,7′-difluorescein, Diaminofluorescein-FM

  • CAS Number 254109-20-1

  • Empirical Formula (Hill Notation) C21H14F2N2O5

  • Molecular Weight 412.34

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Properties

Related Categories Bioactive Small Molecule Alphabetical Index, Bioactive Small Molecules, Cell Biology, Cell Signaling and Neuroscience, D-DIF,
assay   ≥98% (HPLC)
form   powder
storage condition   desiccated
color   white to brown
solubility   DMSO: >1 mg/mL
shipped in   wet ice
storage temp.   −20°C

Description

Caution

hygroscopic

Preparation Note

Color variation within the range from white to brown does not change performance.

Biochem/physiol Actions

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.

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Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Nitric Oxide Synthases

Nitric oxide is a reactive free radical gas that can act as an intracellular or extracellular messenger. It may act locally as an autacoid, paracrine substance or neurotransmitter, and at a distant t...
Keywords: Adhesion, Angiogenesis, Antimicrobials, Biological processes, Cell signaling, Gene expression, Inflammation, Metabolism, Myristoylations, Neurotransmission, Neurotransmitters, Oxidations, Palmitoylations, Phosphorylations

Sensor Dyes

In 1998 R.F. Furchgott, L.J. Ignarro, and F. Murad received the Nobel Prize in Physiology and Medicine for their discoveries concerning nitric oxide as a signaling molecule in the cardiovascular syst...
Monika Baeumle
BioFiles 2011, 6.3, 23.
Keywords: Applications, Biochemistry, Biological processes, Cancer, Cardiovascular, Central Nervous System, Diseases, Extinction coefficient, Indicators, Inflammation, Methods, Microscopy, Neurotransmission, Sol-gel, Type

Peer-Reviewed Papers
15

References

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