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  • D2178 - Monoclonal Anti-Death Associated Protein Kinase antibody produced in mouse

D2178 Sigma

Monoclonal Anti-Death Associated Protein Kinase antibody produced in mouse

clone DAPK-55, ascites fluid

Synonym: Anti-DAP Kinase


Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Other Apoptosis-related Proteins,
species reactivity   monkey, human
application(s)   immunoprecipitation: suitable
  microarray: suitable
  western blot: 1:250 using COS-7 cell extract
clone   DAPK-55, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 160 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... DAP(1611)
biological source   mouse
conjugate   unconjugated



recombinant human DAP kinase expressed in bacteria.

General description

The gene is widely expressed in many tissues of embryonic and adult origin and gives rise to a single 6.3 Kb mRNA transcript. The protein consists of 1423 amino acids (160 kDa) and belongs to a family of calmodulin-dependent protein kinases. It contains 8 ankyrin repeats and 2 potential P-loop motifs apart from the kinase domain and the calmodulin regulatory domain.


Monoclonal Anti-Death Associated Protein Kinase antibody produced in mouse is suitable for immunoprecipitation, microarray and western blotting at a working dilution of 1:250 using COS-7 cell extract. It may be used for the detection and quantification of kinase activity of DAP Kinase using various immunochemical assays such as immunoblot and immunoprecipitation.

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Biochem/physiol Actions

DAP-kinase is a novel, structurally unique, human serine/threonine kinase identified as a positive mediator of interferon-γ-induced programmed cell death. It has an intrinsic kinase activity towards itself that is regulated by calmodulin and calcium.

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